Anti-Copper-Transporting ATPase1 Antibody | QED Bioscience 56516 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Copper-Transporting ATPase1 Antibody

catalog :

56516

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S60-4

The same clone is also sold as:

Novus Biologicals: NBP2-59376
OriGene: AM60002PU-N

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56516

PRODUCT NAME :

Anti-Copper-Transporting ATPase1 Antibody

NAME+SKU :

Anti-Copper-Transporting ATPase1 Antibody (56516)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Copper-Transporting ATPase1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Copper-Transporting ATPase1

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Copper-Transporting ATPase1

UNIPROT :

Q04656

LOCATION :

Golgi apparatus, trans-Golgi network membrane , Cell membrane , Melanosome membrane , Early endosome membrane , Cell projection, axon , Cell projection, dendrite , Cell junction, synapse, postsynaptic density

PROTEIN NAME :

Copper-transporting ATPase 1

ALTERNATIVE NAMES :

EC 7.2.2.8, Copper pump 1, Menkes disease-associated protein

GENE NAME :

ATP7A

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56516

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-2ug/ml. A band of ~180kDa is detected.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2b

CLONE ID :

S60-4

IMMUNOGEN :

Synthetic peptide correspond- ing to aa 42-61 (cytoplasmic C-terminus) of human Copper-Transporting ATPase1 (accession no. NP_000043.3).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7A (also known as Copper-Transporting ATPase1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Although ATP7A is not detectable in most normal tissues, it is expressed in many common tumor types. Increased expression of ATP7A renders tumor cells resistant to cisplatin and carboplatin.

FUNCTION :

ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis (PubMed:10419525, PubMed:11092760, PubMed:28389643). Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:10419525, PubMed:19453293, PubMed:19917612, PubMed:31283225, PubMed:28389643). Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway (PubMed:28389643, PubMed:11092760). Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions (PubMed:10419525, PubMed:28389643). May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3 (PubMed:28389643) (By similarity). In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis (By similarity). {UniProtKB:Q64430, PubMed:10419525, PubMed:11092760, PubMed:19453293, PubMed:19917612, PubMed:28389643, PubMed:31283225}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, and rat Copper-Transporting ATPase1.Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations.Enduser should determine optimal concentrations for their

Image Name_1 :

qed-bioscience-anti-copper-transporting-atpase1-antibody-56516-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-copper-transporting-atpase1-antibody-56516-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-copper-transporting-atpase1-antibody-56516-3.png

Applications 3 :

Western Blot