Anti-VGlut1 Transporter Antibody | QED Bioscience 56514 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-VGlut1 Transporter Antibody

catalog :

56514

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S28-9

The same clone is also sold as:

OriGene: TA326591
Novus Biologicals: NBP2-59329

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56514

PRODUCT NAME :

Anti-VGlut1 Transporter Antibody

NAME+SKU :

Anti-VGlut1 Transporter Antibody (56514)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-VGlut1 Transporter Mouse Monoclonal Antibody

SPECIFICITY SHORT :

VGlut1 Transporter

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

VGlut1 Transporter

UNIPROT :

Q62634

LOCATION :

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane , Membrane , Cell junction, synapse, synaptosome

PROTEIN NAME :

Vesicular glutamate transporter 1

ALTERNATIVE NAMES :

VGluT1, Brain-specific Na(+-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

GENE NAME :

Slc17a7

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56514

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-2ug/ml. A band of ~52kDa is detected. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain lysate.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S28-9

IMMUNOGEN :

Fusion protein corresponding to aa 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession no. NP_446311).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

VGlut1 is expressed in a subset of glutamate neurons where it transports glutamate into native synaptic vesicles from the brain. Glutamate transport by VGlut1 is saturated with a K(m) of approximately 2mM. Whereas plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, VGlut1 recognizes only glutamate.

FUNCTION :

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {PubMed:10938000, PubMed:11001057, PubMed:11698619, PubMed:11698620, PubMed:18080752, PubMed:8202535}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, and rat VGlut1. It does not cross-react with VGlut2.

Image Name_1 :

qed-bioscience-anti-vglut1-transporter-antibody-56514-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-vglut1-transporter-antibody-56514-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-vglut1-transporter-antibody-56514-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-vglut1-transporter-antibody-56514-4.png

Image Caption_4 :

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (56514). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (56514) at 1:1000.

Applications 4 :

Western Blot