This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-Piccolo Antibody
catalog :
56484
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6H9-B6

The same clone is also sold as:
reactivity :
human, mouse, rat
product information
CATALOG NUMBER :
56484
PRODUCT NAME :
Anti-Piccolo Antibody
NAME+SKU :
Anti-Piccolo Antibody (56484)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-Piccolo Mouse Monoclonal Antibody
SPECIFICITY SHORT :
Piccolo
SPECIES REACTIVITY :
Human, rat
TARGET NAME :
Piccolo
UNIPROT :
Q9QYX7
LOCATION :
Cell junction, synapse, presynaptic active zone
PROTEIN NAME :
Protein piccolo
ALTERNATIVE NAMES :
Aczonin, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein
GENE NAME :
Pclo
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
264.6
SKU SIZE 1 :
56484
APPLICATIONS :
WB, ELISA
APPLICATION INSTRUCTIONS :
Immunoblotting: use at 1-10ug/ml. A band of ~550kDa is detected. ELISA: use at 1-10ug/ml with Piccolo on the solid phase. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG2b
CLONE ID :
6H9-B6
IMMUNOGEN :
Full-length human Piccolo protein (accession no. BC001304.1)
RESEARCH AREA :
Neuroscience
FORMULATION :
PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
BUFFER ANTI-MICROBIAL :
0.09% Sodium Azide
BUFFER CRYOPRESERVATIVE :
50% Glycerol
TARGET BACKGROUND :
Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin- mediated endocytosis by binding to the F- actin and dynamin bind protein Abp1.
FUNCTION :
Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:Q9JKS6, PubMed:19812333, PubMed:28231469}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
SPECIFICITY :
This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States