Anti-Beta 3 GABA-A Receptor Antibody | QED Bioscience 56482 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Beta 3 GABA-A Receptor Antibody

catalog :

56482

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S87-25

The same clone is also sold as:

OriGene: TA326474
Rockland Immunochemicals: 200-301-F32

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56482

PRODUCT NAME :

Anti-Beta 3 GABA-A Receptor Antibody

NAME+SKU :

Anti-Beta 3 GABA-A Receptor Antibody (56482)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Beta 3 GABA-A Receptor Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Beta 3 GABA-A Receptor

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Beta 3 GABA-A Receptor

UNIPROT :

P63080

LOCATION :

Cell junction, synapse, postsynaptic cell membrane , Cell membrane , Cytoplasmic vesicle membrane

PROTEIN NAME :

Gamma-aminobutyric acid receptor subunit beta-3

ALTERNATIVE NAMES :

GABA(A receptor subunit beta-3

GENE NAME :

Gabrb3

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56482

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~55kDa is detected. Immunohistochemistry: use at 1-10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S87-25

IMMUNOGEN :

Fusion protein corresponding to aa 370-433 of mouse Beta 3 GABA-A receptor (accession no. AAB60502).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins thathelp establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. In some ion channels, the flow of ions is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. The GABA-A receptor is a fast acting ligand-gated ion channel. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain.

FUNCTION :

Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447). {UniProtKB:P28472, PubMed:10670447, PubMed:27129275, PubMed:9108119}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rat Beta 3 GABA-A receptor. It does not cross-react with Beta 1 or 2 GABA-A receptor.

Image Name_1 :

qed-bioscience-anti-beta-3-gaba-a-receptor-antibody-56482-1.png

Applications 1 :

IHC

Image Name_2 :

qed-bioscience-anti-beta-3-gaba-a-receptor-antibody-56482-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-beta-3-gaba-a-receptor-antibody-56482-3.png

Applications 3 :

ICC