This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-Rhodopsin Antibody
catalog :
56474
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D4

The same clone is also sold as:
reactivity :
bovine
product information
CATALOG NUMBER :
56474
PRODUCT NAME :
Anti-Rhodopsin Antibody
NAME+SKU :
Anti-Rhodopsin Antibody (56474)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-Rhodopsin Mouse Monoclonal Antibody
SPECIFICITY SHORT :
Rhodopsin
SPECIES REACTIVITY :
Vertebrate
TARGET NAME :
Rhodopsin
UNIPROT :
P02699
LOCATION :
Membrane , Cell projection, cilium, photoreceptor outer segment
PROTEIN NAME :
Rhodopsin
GENE NAME :
RHO
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
305.2
SKU SIZE 1 :
56474
APPLICATIONS :
WB, IHC, ICC, IP
APPLICATION INSTRUCTIONS :
Immunoblotting: use at 1-10ug/ml. A band of ~40kDa is detected. Immunohistochemistry/immunocyto- chemistry: use at 1-10ug/ml Immunoprecipitation: use at 1-2ug per 100-500ug of protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat eye lysate
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG1
CLONE ID :
1D4
IMMUNOGEN :
Bovine rhodopsin
RESEARCH AREA :
Neuroscience
FORMULATION :
PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
BUFFER ANTI-MICROBIAL :
0.09% Sodium Azide
BUFFER CRYOPRESERVATIVE :
50% Glycerol
TARGET BACKGROUND :
Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.
FUNCTION :
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
SPECIFICITY :
This antibody recognizes the C-terminal aa TETSQVAPA(COOH) of native and recombinant vertebrate rhodopsin.
Image Name_1 :
qed-bioscience-anti-rhodopsin-antibody-56474-1.png
Applications 1 :
IHC
Image Name_2 :
qed-bioscience-anti-rhodopsin-antibody-56474-2.png
Applications 2 :
Western Blot
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States