Anti-NR2B Glutamate Receptor Antibody | QED Bioscience 56461 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-NR2B Glutamate Receptor Antibody

catalog :

56461

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S59-36

The same clone is also sold as:

Abcam: ab93610
Rockland Immunochemicals: 200-301-G23
MyBioSource: MBS800075, MBS246120
OriGene: TA309448

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56461

PRODUCT NAME :

Anti-NR2B Glutamate Receptor Antibody

NAME+SKU :

Anti-NR2B Glutamate Receptor Antibody (56461)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-NR2B Glutamate Receptor Mouse Monoclonal Antibody

SPECIFICITY SHORT :

NR2B Glutamate Receptor

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

NR2B Glutamate Receptor

UNIPROT :

Q00960

LOCATION :

Cell membrane , Cell junction, synapse, postsynaptic cell membrane , Late endosome , Lysosome , Cytoplasm, cytoskeleton

PROTEIN NAME :

Glutamate receptor ionotropic, NMDA 2B

ALTERNATIVE NAMES :

GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B

GENE NAME :

Grin2b

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56461

APPLICATIONS :

WB, IHC, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~166kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml Immunofluorescence: use at 1-10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2b

CLONE ID :

S59-36

IMMUNOGEN :

Fusion protein aa 20-271 (extracellular N-terminus) of rat NR2B (accession no. Q00960). This sequence is 99

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Glutamate receptors of the NMDA subtype are involved in several physiological and pathological processes in the brain. Functional NMDA receptors are heteromultimeric complexes of the NR1 subunit and one or more of the four NR2 subunits (NR2A-D). Coexpression of both subunit types are required for the for- mation of fully functional channels that are inserted into the plasma membrane. The NR2 subunit determines characteristics of the channel such as conductance, mean open time, and sensitivity to Mg+2. The ability of NR1 and NR2 subunit combinations to flux Ca+2 is essential to the role that NMDA receptors play in synaptic plasticity and neurotoxicity.

FUNCTION :

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1350383, PubMed:19910922, PubMed:21677647, PubMed:24876489, PubMed:27135925, PubMed:27916457). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity). {UniProtKB:Q01097, PubMed:1350383, PubMed:19910922, PubMed:21677647, PubMed:24876489, PubMed:27135925, PubMed:27916457, ECO:0000305}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, and rat NR2B. It does not cross-react with NR2A.

Image Name_1 :

qed-bioscience-anti-nr2b-glutamate-receptor-antibody-56461-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-nr2b-glutamate-receptor-antibody-56461-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-nr2b-glutamate-receptor-antibody-56461-3.png

Applications 3 :

IHC

Image Name_5 :

qed-bioscience-anti-nr2b-glutamate-receptor-antibody-56461-5.png

Image Caption_5 :

Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 (56461). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (56461) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 5 :

Western Blot