Anti-PSD95 Antibody | QED Bioscience 56457 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-PSD95 Antibody

catalog :

56457

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7E3-1B8

The same clone is also sold as:

Invitrogen: MA1-046
Abcam: ab13552

reactivity :

mouse, rat, bovine

product information

CATALOG NUMBER :

56457

PRODUCT NAME :

Anti-PSD95 Antibody

NAME+SKU :

Anti-PSD95 Antibody (56457)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-PSD95 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

PSD95

SPECIES REACTIVITY :

Mouse, rat, bovine

TARGET NAME :

PSD95

UNIPROT :

P31016

LOCATION :

Cell membrane , Cell junction, synapse, postsynaptic density , Cell junction, synapse , Cytoplasm , Cell projection, axon , Cell projection, dendritic spine , Cell projection, dendrite , Cell junction, synapse, presynapse

PROTEIN NAME :

Disks large homolog 4

ALTERNATIVE NAMES :

Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90

GENE NAME :

Dlg4

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

298.9

SKU SIZE 1 :

56457

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. A band of ~100 kDa is detected. Immunocytochemistry: use at 1-10ug/ml. Immunofluorescence: use at10ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

7E3-1B8

IMMUNOGEN :

Recombinant rat PSD95

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Postsynaptic Density Protein 95 (PSD95), also known as Synapse Associated Protein 90kDa, is a member of the membrane-associated guanylate kinase family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex. This family of proteins consists of N-terminal variable segments followed by three amino-terminal PDZ domains, an upstream SH3 domain, and an inactive carboxy-terminal guanylate kinase domain. The first and second PDZ domains localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins, neuronal cell adhesion molecules. PSD95 also binds to neuronal nitric oxide synthase. PSD95 is implicated in experience-dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.

FUNCTION :

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (PubMed:19596852).Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity). {UniProtKB:P78352, UniProtKB:Q62108, PubMed:15317815, PubMed:15358863, PubMed:19596852, PubMed:23300088, PubMed:26679993}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes mouse, rat, pig, and human PSD95.

Image Name_1 :

qed-bioscience-anti-psd95-antibody-56457-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-psd95-antibody-56457-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-psd95-antibody-56457-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-psd95-antibody-56457-4.png

Image Caption_4 :

Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 (56457). Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (56457) at 1:1000.

Applications 4 :

Western Blot