Anti-CaMKII, phosphorylated, Antibody | QED Bioscience 56454 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-CaMKII, phosphorylated, Antibody

catalog :

56454

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

22B1

The same clone is also sold as:

Rockland Immunochemicals: 200-301-F01
Santa Cruz Biotechnology: sc-32289, sc-32289 AC, sc-32289 HRP
Invitrogen: MA5-45146, MA5-45148, MA5-45147, MA1-047
Novus Biologicals: NB110-96869
StressMarq Biosciences: SMC-125
OriGene: TA326521
Abcam: ab171095

reactivity :

mouse, rat

product information

CATALOG NUMBER :

56454

PRODUCT NAME :

Anti-CaMKII, phosphorylated, Antibody

NAME+SKU :

Anti-CaMKII, phosphorylated, Antibody (56454)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-CamKII, phosphorylated Mouse Monoclonal Antibody

SPECIFICITY SHORT :

CamKII, phosphorylated

SPECIES REACTIVITY :

Mouse, rat

TARGET NAME :

CaMKII, phosphorylated,

UNIPROT :

P11275

LOCATION :

Cell junction, synapse , Cell junction, synapse, postsynaptic density , Cell projection, dendritic spine , Cell projection, dendrite

PROTEIN NAME :

Calcium/calmodulin-dependent protein kinase type II subunit alpha

ALTERNATIVE NAMES :

CaM kinase II subunit alpha, CaMK-II subunit alpha, EC 2.7.11.17

GENE NAME :

Camk2a

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

298.9

SKU SIZE 1 :

56454

APPLICATIONS :

WB, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. A band of ~50 kDa is detected. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

22B1

IMMUNOGEN :

Synthetic peptide based on sequence from rat/mouse CaMKII alpha (aa 281-294), thiophosphorylated at residue Thr286 conjugated to a protein carrier.

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto- inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.

FUNCTION :

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (PubMed:15312654). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (By similarity). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity). {UniProtKB:P11798, UniProtKB:Q9UQM7, PubMed:15312654}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes the phosphorylated form of mouse and rat CaMKII. It does not react with non- phosphorylated CaMKII.

Image Name_1 :

qed-bioscience-anti-camkii-phosphorylated-antibody--56454-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-camkii-phosphorylated-antibody--56454-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-camkii-phosphorylated-antibody--56454-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-camkii-phosphorylated-antibody--56454-4.png

Image Caption_4 :

Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (56454). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56454) at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

Applications 4 :

Western Blot