This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-CaMKII Antibody
catalog :
56453
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6G9

The same clone is also sold as:
reactivity :
mouse, rat, bovine
product information
CATALOG NUMBER :
56453
PRODUCT NAME :
Anti-CaMKII Antibody
NAME+SKU :
Anti-CaMKII Antibody (56453)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-CaMKII Mouse Monoclonal Antibody
SPECIFICITY SHORT :
CaMKII
SPECIES REACTIVITY :
Mouse, rat, bovine
TARGET NAME :
CaMKII
UNIPROT :
P11798
LOCATION :
[Isoform Alpha KAP]: Cytoplasm , Cell junction, synapse, postsynaptic density , Cell projection, dendritic spine , Cell projection, dendrite
PROTEIN NAME :
Calcium/calmodulin-dependent protein kinase type II subunit alpha
ALTERNATIVE NAMES :
CaM kinase II subunit alpha, CaMK-II subunit alpha, EC 2.7.11.17
GENE NAME :
Camk2a
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
298.9
SKU SIZE 1 :
56453
APPLICATIONS :
WB, IHC, RIA
APPLICATION INSTRUCTIONS :
Immunoblotting: use at 0.1-1ug/ml. A band of ~50-60 kDa is detected. Immunohistochemistry: use at 10ug/ml. RIA: use at 3-5ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG1
CLONE ID :
6G9
IMMUNOGEN :
Partially purified rat CaMKII
RESEARCH AREA :
Neuroscience
FORMULATION :
PBS, pH 7.4.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
TARGET BACKGROUND :
CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto- inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.
FUNCTION :
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development. Also regulates the migration of developing neurons (By similarity). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (PubMed:23502535). {UniProtKB:P11275, UniProtKB:Q9UQM7, PubMed:23502535, PubMed:23805378}.; [Isoform Alpha KAP]: Has no kinase activity. {PubMed:8524307}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
SPECIFICITY :
This antibody recognizes the phosphorylated and non-phosphorylated forms of mouse, rat, and bovine CaMKII.
Image Name_1 :
qed-bioscience-anti-camkii-antibody-56453-1.png
Applications 1 :
ICC
Image Name_2 :
qed-bioscience-anti-camkii-antibody-56453-2.png
Applications 2 :
ICC
Image Name_3 :
qed-bioscience-anti-camkii-antibody-56453-3.png
Applications 3 :
IHC
Image Name_4 :
qed-bioscience-anti-camkii-antibody-56453-4.png
Image Caption_4 :
Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.
Applications 4 :
IHC
Image Name_5 :
qed-bioscience-anti-camkii-antibody-56453-5.png
Image Caption_5 :
Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:1000.
Applications 5 :
Western Blot
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States