This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-CD74 Antibody
catalog :
56451
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PIN1

The same clone is also sold as:
reactivity :
human
product information
CATALOG NUMBER :
56451
PRODUCT NAME :
Anti-CD74 Antibody
NAME+SKU :
Anti-CD74 Antibody (56451)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-CD74 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
CD74
SPECIES REACTIVITY :
Human
TARGET NAME :
CD74
UNIPROT :
P04233
LOCATION :
Cell membrane , Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.; [Isoform p41]: Late endosome , Lysosome
PROTEIN NAME :
HLA class II histocompatibility antigen gamma chain
ALTERNATIVE NAMES :
HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, CD antigen CD74 [Cleaved into: Class-II-associated invariant chain peptide
GENE NAME :
CD74
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
259.7
SKU SIZE 1 :
56451
APPLICATIONS :
WB, IP, ICC, FACS
APPLICATION INSTRUCTIONS :
Immunoblotting: use at 1ug/ml. A doublet band of ~33-35 kDa is detected. Immunoprecipitation: use at 12ug/ml. Immunocytochemistry: working concentration is sample dependent. Flow cytometry: working concentration is sample dependent. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: PALA cell lysate.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG1
CLONE ID :
PIN1
IMMUNOGEN :
Synthetic peptide corresponding to the human CD74 invariant chain (aa 12-28) conjugated to KLH.
RESEARCH AREA :
Neuroscience
FORMULATION :
PBS, pH 7.4.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
TARGET BACKGROUND :
CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. CD74 functions mainly as an MHC class II chaperone which promotes ER exit of MHC class II molecules by directing them to endocytic compartments, preventing peptide binding in the ER, and contributing to peptide “editing” in the MHC class II compartment. CD74 has recently been shown to have a role as an accessory signaling molecule because of its high- affinity binding to the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).
FUNCTION :
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.; [Class-II-associated invariant chain peptide]: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. {PubMed:1448172}.; [Isoform p41]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215). {UniProtKB:P04441, PubMed:32855215}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
SPECIFICITY :
This antibody recognizes human CD74.
Image Name_1 :
qed-bioscience-anti-cd74-antibody-56451-1.png
Applications 1 :
ICC
Image Name_2 :
qed-bioscience-anti-cd74-antibody-56451-2.png
Applications 2 :
ICC
Image Name_3 :
qed-bioscience-anti-cd74-antibody-56451-3.png
Applications 3 :
IHC
Image Name_4 :
qed-bioscience-anti-cd74-antibody-56451-4.png
Image Caption_4 :
Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (56451). Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (56451) at 1:1000. Lysates treated with macrophage inhibitory factor (MIF). Courtesy of: Victor E. Reyes, University of Texas Medical Branch, USA.
Applications 4 :
Western Blot
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States