Anti-LAMP2 Antibody | QED Bioscience 56272 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-LAMP2 Antibody

catalog :

56272

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

GL2A7

The same clone is also sold as:

OriGene: TA309434
StressMarq Biosciences: SMC-141
Developmental Studies Hybridoma Bank: GL2A7
Rockland Immunochemicals: 200-501-G10
Invitrogen: MA5-45169, MA1-165, MA5-45171, MA5-45170
Abcam: ab13524

reactivity :

mouse

product information

CATALOG NUMBER :

56272

PRODUCT NAME :

Anti-LAMP2 Antibody

NAME+SKU :

Anti-LAMP2 Antibody (56272)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-LAMP2 Antibody Rat Monoclonal Antibody

SPECIFICITY SHORT :

LAMP2 Antibody

SPECIES REACTIVITY :

Mouse, rabbit

TARGET NAME :

LAMP2

UNIPROT :

P17047

LOCATION :

Cell membrane , Endosome membrane , Cytoplasmic vesicle, autophagosome membrane , Lysosome membrane

PROTEIN NAME :

Lysosome-associated membrane glycoprotein 2

ALTERNATIVE NAMES :

LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B, CD antigen CD107b

GENE NAME :

Lamp2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

293.3

SKU SIZE 1 :

56272

APPLICATIONS :

ICC, IP

APPLICATION INSTRUCTIONS :

Immunoblotting: Use at 1-2ug/ml. A band of ~100-110kDa (glycosylated) is detected. Immunofluorescence: Use at 1-2ug/ml. This antibody labels lysosomes and late endosomes in cells permeabilized with saponin. Lane 1: MW ladder, Lane 2: 20ug HEK293 lysate, Lane 3: 10ug 3T3NIH lysate These are recommended concentrations. Enduser should determine optimal concentrations for their application.

HOST :

Rat

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

GL2A7

IMMUNOGEN :

Gluteraldehyde-fixed mouse liver lysosomes

RESEARCH AREA :

Cell adhesion

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Lysosome-associated membrane proteins (LAMP1 and LAMP2) are major constituents of the lysosomal membrane. These two proteins have closely related structures with 37% sequence homology. Both are transmembrane glycoproteins localized primarily in lysosomes and late endosomes. LAMP2 has also been detected at the plasma membrane of cells undergoing differentiation and activation and in cells that secrete lysosomal hydrolases. Cell surface LAMP1 and LAMP2 promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium which suggests that the LAMP proteins are involved in adhesion of PBMC to sites of inflammation. Defects in LAMP2 are associated with Danon disease.

FUNCTION :

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:10972293). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:10972293, PubMed:12221139). Plays a role in lysosomal protein degradation in response to starvation (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity). {UniProtKB:P13473, UniProtKB:P17046, PubMed:10972293, PubMed:12221139, PubMed:27628032}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rabbit LAMP2 (~100-110 kDa).Accession no.: NP_001017959.1 Gene ID: 16784

Image Name_1 :

qed-bioscience-anti-lamp2-antibody-56272-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-lamp2-antibody-56272-2.png

Applications 2 :

Western Blot