Anti-DNA Ligase III Antibody | QED Bioscience 56250 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-DNA Ligase III Antibody

catalog :

56250

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F3

The same clone is also sold as:

Novus Biologicals: NBP1-41190
Invitrogen: MA1-23191
GeneTex: GTX70143

reactivity :

human, mouse, chicken

product information

CATALOG NUMBER :

56250

PRODUCT NAME :

Anti-DNA Ligase III Antibody

NAME+SKU :

Anti-DNA Ligase III Antibody (56250)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-DNA Ligase III Mouse Monoclonal Antibody

SPECIFICITY SHORT :

DNA Ligase III

SPECIES REACTIVITY :

Human, mouse, chicken

TARGET NAME :

DNA Ligase III

UNIPROT :

P49916

LOCATION :

[Isoform 1]: Mitochondrion

PROTEIN NAME :

DNA ligase 3

ALTERNATIVE NAMES :

EC 6.5.1.1, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3

GENE NAME :

LIG3

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

56250

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.5-2ug/ml. Predicted molecular weight ~100kDa. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

1F3

IMMUNOGEN :

Full-length recombinant human DNA Ligase III-b.

RESEARCH AREA :

Cancer research

FORMULATION :

Hybridoma culture supernatant

BUFFER :

Hybridoma culture supernatant

TARGET BACKGROUND :

There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97kDa (Â§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway.

FUNCTION :

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {PubMed:10207110, PubMed:24674627}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, and chicken DNA Ligase III. Cellular localization is nuclear and mitochondrial.

Image Name_1 :

qed-bioscience-anti-dna-ligase-iii-antibody-56250-1.jpg

Applications 1 :

Western Blot

Image Name_2 :

qed-bioscience-anti-dna-ligase-iii-antibody-56250-2.jpg

Applications 2 :

Western Blot