This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-p84 Antibody
catalog :
56248
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5E10

The same clone is also sold as:
reactivity :
human
product information
CATALOG NUMBER :
56248
PRODUCT NAME :
Anti-p84 Antibody
NAME+SKU :
Anti-p84 Antibody (56248)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-p84 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
p84
SPECIES REACTIVITY :
Human
TARGET NAME :
p84
UNIPROT :
Q96FV9
LOCATION :
[Isoform 1]: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.; [Isoform 2]: Cytoplasm.
PROTEIN NAME :
THO complex subunit 1
ALTERNATIVE NAMES :
Tho1, Nuclear matrix protein p84, p84N5, hTREX84
GENE NAME :
THOC1
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
322.7
SKU SIZE 1 :
56248
APPLICATIONS :
WB, IP, IF
APPLICATION INSTRUCTIONS :
Immunoblotting, immunoprecipitation, and immunofluorescence: use at 0.5-2 ug/ml. In immunoblots, a band of 84 kD is detected. Positive controls: Lysates of Molt 4, HeLa, or Raji cells.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by Protein G affinity chromatography.
ISOTYPE :
IgG2b
CLONE ID :
5E10
IMMUNOGEN :
Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli.
RESEARCH AREA :
Cancer research
FORMULATION :
PBS, pH 7.4.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
FUNCTION :
Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.; Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.
SPECIFICITY :
This antibody recognizes an 84 kD nuclear matrix protein that was first discovered through its association with the N-terminal region of retinoblastoma protein.
Image Name_1 :
qed-bioscience-anti-p84-antibody-56248-1.jpg
Applications 1 :
IHC
Image Name_2 :
qed-bioscience-anti-p84-antibody-56248-2.jpg
Applications 2 :
Western Blot
Image Name_3 :
qed-bioscience-anti-p84-antibody-56248-3.jpg
Applications 3 :
IF
Image Name_4 :
qed-bioscience-anti-p84-antibody-56248-4.jpg
Image Caption_4 :
p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (56248) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (56248) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Applications 4 :
WB
Image Name_5 :
qed-bioscience-anti-p84-antibody-56248-5.jpg
Image Caption_5 :
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7.5% SDS PAGE 56248 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Applications 5 :
WB
Image Name_6 :
qed-bioscience-anti-p84-antibody-56248-6.jpg
Image Caption_6 :
Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7.5% SDS PAGE 56248 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Applications 6 :
WB
Image Name_7 :
qed-bioscience-anti-p84-antibody-56248-7.jpg
Image Caption_7 :
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE 56248 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Applications 7 :
WB
Image Name_8 :
qed-bioscience-anti-p84-antibody-56248-8.jpg
Image Caption_8 :
Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (56248) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications 8 :
IHC
Image Name_9 :
qed-bioscience-anti-p84-antibody-56248-9.jpg
Image Caption_9 :
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (56248) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Applications 9 :
WB
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States