Anti-RPA32 Antibody | QED Bioscience 56216 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-RPA32 Antibody

catalog :

56216

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12F3.3

The same clone is also sold as:

Invitrogen: MA1-23284
Novus Biologicals: NB100-158

reactivity :

human, mouse

product information

CATALOG NUMBER :

56216

PRODUCT NAME :

Anti-RPA32 Antibody

NAME+SKU :

Anti-RPA32 Antibody (56216)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-RPA32 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

RPA32

SPECIES REACTIVITY :

Human, mouse

TARGET NAME :

RPA32

UNIPROT :

P15927

LOCATION :

Nucleus , Nucleus, PML body

PROTEIN NAME :

Replication protein A 32 kDa subunit

ALTERNATIVE NAMES :

RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34

GENE NAME :

RPA2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

56216

APPLICATIONS :

WB, IP

APPLICATION INSTRUCTIONS :

Immunoblotting, immunoprecipitation: use at 0.2-1 ug/ml. Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

12F3.3

IMMUNOGEN :

N-terminally 6x-His tagged fusion protein corresponding to full-length human RPA32 expressed in E. coli.

RESEARCH AREA :

Cancer research

FORMULATION :

PBS, pH 7.4

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. {PubMed:15205463, PubMed:17765923, PubMed:17959650, PubMed:19116208, PubMed:20154705, PubMed:21504906, PubMed:2406247, PubMed:24332808, PubMed:7697716, PubMed:7700386, PubMed:8702565, PubMed:9430682, PubMed:9765279}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human and mouse RPA32. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair. RPA32 is phosphorylated during the S-phase of the cell cycle, in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes.

Image Name_1 :

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Applications 1 :

Image Name_2 :

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Applications 2 :

Image Name_3 :

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Applications 3 :

Western Blot