This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-Rad51 Antibody
catalog :
56214
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
14B4

The same clone is also sold as:
reactivity :
human
product information
CATALOG NUMBER :
56214
PRODUCT NAME :
Anti-Rad51 Antibody
NAME+SKU :
Anti-Rad51 Antibody (56214)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-Rad51 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
Rad51
SPECIES REACTIVITY :
Human
TARGET NAME :
Rad51
UNIPROT :
Q06609
LOCATION :
Nucleus , Cytoplasm , Cytoplasm, perinuclear region. Mitochondrion matrix , Chromosome , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
PROTEIN NAME :
DNA repair protein RAD51 homolog 1
ALTERNATIVE NAMES :
HsRAD51, hRAD51, RAD51 homolog A
GENE NAME :
RAD51
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
322.7
SKU SIZE 1 :
56214
APPLICATIONS :
WB, IP
APPLICATION INSTRUCTIONS :
Immunoblotting, immunoprecipitation: use at 1-5 ug/ml. Positive control: T24 bladder carcinoma cells and recombinant fusion protein.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG2b
CLONE ID :
14B4
IMMUNOGEN :
GST fusion protein corresponding to full-length Rad51 (aa 1-338) of human Rad51 expressed in E. coli.
RESEARCH AREA :
Cancer research
FORMULATION :
PBS, pH 7.4
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
FUNCTION :
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous rPubMed:12205100, PubMed:12442171, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:20413593, PubMed:23509288, PubMed:23754376, PubMed:26253028, PubMed:26681308, PubMed:28575658}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.
SPECIFICITY :
This antibody recognizes human Rad51, a 37 kD protein that interacts with BRCA1. This interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact via the BRC repeats located with exon 11 of BRCA2. When these repeats are deleted, the interaction is lost, and cells become hypersensitive to DNA damage caused by methyl methanesulfonate (MMS).
Image Name_1 :
qed-bioscience-anti-rad51-antibody-56214-1.jpg
Applications 1 :
WB
Image Name_2 :
qed-bioscience-anti-rad51-antibody-56214-2.jpg
Applications 2 :
WB
Image Name_3 :
qed-bioscience-anti-rad51-antibody-56214-3.jpg
Applications 3 :
IHC
Image Name_4 :
qed-bioscience-anti-rad51-antibody-56214-4.jpg
Image Caption_4 :
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Applications 4 :
WB
Image Name_5 :
qed-bioscience-anti-rad51-antibody-56214-5.jpg
Image Caption_5 :
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Applications 5 :
WB
Image Name_6 :
qed-bioscience-anti-rad51-antibody-56214-6.jpg
Image Caption_6 :
Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Applications 6 :
WB
Image Name_7 :
qed-bioscience-anti-rad51-antibody-56214-7.jpg
Image Caption_7 :
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Applications 7 :
WB
Image Name_8 :
qed-bioscience-anti-rad51-antibody-56214-8.jpg
Image Caption_8 :
Rad51 antibody [14B4] detects Rad51 protein at nuclear foci by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad51 stained by Rad51 antibody [14B4] (56214) diluted at 1:500. Blue: Fluoroshield with DAPI.
Applications 8 :
IF
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States