This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-Fen-1 Antibody
catalog :
56208
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4E7

The same clone is also sold as:
reactivity :
human
product information
CATALOG NUMBER :
56208
PRODUCT NAME :
Anti-Fen-1 Antibody
NAME+SKU :
Anti-Fen-1 Antibody (56208)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-Fen-1 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
Fen-1
SPECIES REACTIVITY :
Human
TARGET NAME :
Fen-1
UNIPROT :
P39748
LOCATION :
[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion
PROTEIN NAME :
Flap endonuclease 1
ALTERNATIVE NAMES :
FEN-1, EC 3.1.-.-, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1
GENE NAME :
FEN1
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
322.7
SKU SIZE 1 :
56208
APPLICATIONS :
WB
APPLICATION INSTRUCTIONS :
Immunoblotting: use at 1-5 ug/ml. Positive controls: MCF-7 cells and recombinant fusion protein.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG1
CLONE ID :
4E7
IMMUNOGEN :
Recombinant human FEN-1 protein (aa 1-380) purified from baculovirus-infected Sf-9 cells.
RESEARCH AREA :
Neuroscience
FORMULATION :
10 mM PBS, pH 7.4.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
TARGET BACKGROUND :
FEN-1 (Flap Endonuclease and Five Prime Exonuclease) is a 42 kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5’ flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 appears to have important roles in DNA replication and repair and is required in cell-fre DNA replication systems.
FUNCTION :
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {HAMAP-Rule:MF_03140, PubMed:10744741, PubMed:11986308, PubMed:18443037, PubMed:20729856, PubMed:26751069, PubMed:7961795, PubMed:8621570}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze- thaw cycles.
SPECIFICITY :
This antibody recognizes human FEN-1.
Image Name_1 :
qed-bioscience-anti-fen-1-antibody-56208-1.jpg
Applications 1 :
WB
Image Name_2 :
qed-bioscience-anti-fen-1-antibody-56208-2.jpg
Applications 2 :
IF
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States