Anti-cdc34 Antibody | QED Bioscience 56207 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-cdc34 Antibody

catalog :

56207

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14B5

The same clone is also sold as:

Novus Biologicals: NB500-168
Invitrogen: MA1-23174

reactivity :

human

product information

CATALOG NUMBER :

56207

PRODUCT NAME :

Anti-cdc34 Antibody

NAME+SKU :

Anti-cdc34 Antibody (56207)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-cdc34 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

cdc34

SPECIES REACTIVITY :

Human

TARGET NAME :

cdc34

UNIPROT :

P49427

LOCATION :

Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

PROTEIN NAME :

Ubiquitin-conjugating enzyme E2 R1

ALTERNATIVE NAMES :

EC 2.3.2.23,

GENE NAME :

CDC34

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

56207

APPLICATIONS :

WB, IP

APPLICATION INSTRUCTIONS :

Immunoblotting and Immuno-precipitation: use at 1-5 ug/ml. Positive controls: 293 cells and recombinant fusion protein.

HOST :

Mouse

FORMAT :

Purified

ISOTYPE :

IgG1

CLONE ID :

14B5

IMMUNOGEN :

N-terminus 6x-His tagged fusion protein encoding full-length human Cdc34 expressed in E. coli.

RESEARCH AREA :

Neuroscience

FORMULATION :

10 mM PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Cdc 34 is a ubiquitin conjugating enzyme that is essential for the transition from G1 to S phase in Saccharomyces cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In extracts of Xenopus laevis eggs, Cdc34 is required for the onset of DNA replication and appears to function in a multi-protein complex. Cdc34 is highly conserved among vertebrates and probably functions similarly in all eukaryotes to regulate the start of DNA synthesis.

FUNCTION :

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421). {PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421, PubMed:22496338}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C to -70C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human Cdc34.

Image Name_1 :

qed-bioscience-anti-cdc34-antibody-56207-1.jpg

Applications 1 :

Image Name_2 :

qed-bioscience-anti-cdc34-antibody-56207-2.jpg

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-cdc34-antibody-56207-3.jpg

Applications 3 :