Anti-Fibronectin Antibody | QED Bioscience 42042 product information

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product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Fibronectin Antibody

catalog :

42042

quantity :

400 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

FN30-8

reactivity :

human

product information

CATALOG NUMBER :

42042

PRODUCT NAME :

Anti-Fibronectin Antibody

NAME+SKU :

Anti-Fibronectin Antibody (42042)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Human Fibronectin Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Human Fibronectin

SPECIES REACTIVITY :

Human

TARGET NAME :

Fibronectin

UNIPROT :

P02751

LOCATION :

Secreted, extracellular space, extracellular matrix

PROTEIN NAME :

Fibronectin

ALTERNATIVE NAMES :

FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3]

GENE NAME :

FN1

CLONALITY :

Monoclonal

FORM :

Lyophilized

CONCENTRATION :

Lot Specific

SIZE 1 :

400 ug

DISTIRBUTOR PRICE :

328.3

SKU SIZE 1 :

42042

APPLICATIONS :

WB, ELISA, Inhibition

APPLICATION INSTRUCTIONS :

ELISA: 1-10ug/ml with immobilized antigen. Western Blot: 1-10ug/ml, reducing or non-reducing conditions. Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells. Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

ISOTYPE :

IgG1

CLONE ID :

FN30-8

IMMUNOGEN :

Human plasma fibronectin

RESEARCH AREA :

Cell adhesion

FORMULATION :

PBS, pH 7.4, 1% BSA, lyophilized.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER PROTEIN STABILIZER :

1% Bovine Serum Albumin

TARGET BACKGROUND :

Fibronectin is a 440kDa glycoprotein of the extracellular matrix that binds to integrins, collagen, fibrin, and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis.

FUNCTION :

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}.; [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.

DILUTION INSTRUCTIONS :

Dissolve lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).

STORAGE CONDITION :

The stock solution (2.0mg/ml) can be stored in aliquots at -20C for 1 year or can be stored at 4C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes an epitope in the cell-binding domain of human fibronectin and can inhibit cell adhesion.