Anti-Insulin Growth Factor-1 Receptor (IGF-1R) Antibody | QED Bioscience 35102 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Insulin Growth Factor-1 Receptor (IGF-1R) Antibody

catalog :

35102

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H7

The same clone is also sold as:

MyBioSource: MBS311301
Invitrogen: MA1-10854, MA1-81739, MA1-21540, MA5-16465, 17-8849-42, MA1-81548, 53-8849-42
Santa Cruz Biotechnology: sc-461, sc-461 HRP, sc-461 L, sc-461 AC
BD Biosciences: 555998, 555999, 562535, 560934
OriGene: BM2226R, BM2226P, BM2226LE, BM2226PT
Bio-Rad: MCA2344, MCA2344T, MCA2344EL, MCA2344A488, MCA2344PE

reactivity :

human

product information

CATALOG NUMBER :

35102

PRODUCT NAME :

Anti-Insulin Growth Factor-1 Receptor (IGF-1R) Antibody

NAME+SKU :

Anti-Insulin Growth Factor-1 Receptor (IGF-1R) Antibody (35102)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-IGF-1 receptor Mouse Monoclonal Antibody

SPECIFICITY SHORT :

IGF-1 receptor

SPECIES REACTIVITY :

Human

TARGET NAME :

Insulin Growth Factor-1 Receptor (IGF-1R)

UNIPROT :

P08069

LOCATION :

Cell membrane , Single-pass type I membrane protein

PROTEIN NAME :

Insulin-like growth factor 1 receptor

ALTERNATIVE NAMES :

EC 2.7.10.1, Insulin-like growth factor I receptor, IGF-I receptor, CD antigen CD221 [Cleaved into: Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain]

GENE NAME :

IGF1R

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

254.8

SKU SIZE 1 :

35102

APPLICATIONS :

WB, inhibits IGF-1 binding

APPLICATION INSTRUCTIONS :

Use these antibodies in research studies to delineate signal transduction pathways specific for IGFâ€™s and for detecting IGF-1 receptors in crude samples. 35101 may be used to immunoprecipitate IGF-1 receptors. 35102 and 35103 can recognize the IGF-1 alpha subunit on immunoblots.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

1H7

IMMUNOGEN :

Purified human placental IGF-1 receptors.

RESEARCH AREA :

Growth Factors, Cytokines, Receptors

FORMULATION :

PBS, pH 7.4

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.; When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

All three clones are specific for the human IGF-1 receptor; 35102 and 35103 recognize the alpha subunit: 35101 (3B7) stimulates binding of IGF-1 and -2; stimulates basal and IGF- 1 stimulated cell growth. 35102 (1H7) inhibits binding of IGF-1 and -2; inhibits cell growth. 35103 (2C8) has no effect on binding of IGF-1 or -2.