Anti-MLL.N Antibody | QED Bioscience 34041 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-MLL.N Antibody

catalog :

34041

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N4.4

reactivity :

human, mouse

product information

CATALOG NUMBER :

34041

PRODUCT NAME :

Anti-MLL.N Antibody

NAME+SKU :

Anti-MLL.N Antibody (34041)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-MLL (N-terminus) Mouse Monoclonal Antibody

SPECIFICITY SHORT :

MLL (N-terminus)

SPECIES REACTIVITY :

Human, mouse

TARGET NAME :

MLL.N

UNIPROT :

Q9Y6P1

PROTEIN NAME :

MLL

GENE NAME :

MLL

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

289.1

SKU SIZE 1 :

34041

APPLICATIONS :

WB, IP

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 2ug/ml. A band of ~300kDa is detected. Immunoprecipitation: use 2-10ug per 500ul (1-3mg/ml) of cell lysate. Positive control: K562 cell lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

N4.4

IMMUNOGEN :

Maltose-binding fusion protein containing MLL amino acids 161-356 (Uniprot #Q03164).

RESEARCH AREA :

Cancer research

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

MLL (Mixed Lineage Leukemia) is a proto- oncogene that is mutated in a variety of acute leukemias. MLL is proteolytically processed into 2 fragments, MLLN and MLLC, that display opposite transcriptional properties. Processed MLLN and MLLC associate with each other via N-terminal and C-terminal intramolecular interaction domains. MLL processing occurs rapidly after translation and is followed by phosphorylation of MLLC. MLLN displays transcriptional repression activity, whereas MLLC has strong transcriptional activation properties. Leukemia-associated MLL fusion proteins lack the MLL processing sites, do not undergo cleavage, and are unable to interact with MLLC. These observations suggest that posttranslational modifications of MLL may be involved in regulating MLL activity as a transcription factor, and this aspect of its function is perturbed by leukemogenic fusions.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human and mouse MLLN.

Image Name_1 :

qed-bioscience-anti-mll.n-antibody-34041-1.jpg

Applications 1 :