Anti-HIF1 alpha Antibody | QED Bioscience 34039 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-HIF1 alpha Antibody

catalog :

34039

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ESEE122

The same clone is also sold as:

Novus Biologicals: NBP2-80762, NB100-131
StressMarq Biosciences: SMC-184
OriGene: TA309690, TA326476
Invitrogen: MA5-45252, MA5-16020, 14-9100-82, MA5-45251, MA5-16017, 14-9100-80, MA5-16018, MA1-16518, MA5-16019, MA5-16016, MA5-45250
Rockland Immunochemicals: 200-301-F45
Abcam: ab8366

reactivity :

human, mouse

product information

CATALOG NUMBER :

34039

PRODUCT NAME :

Anti-HIF1 alpha Antibody

NAME+SKU :

Anti-HIF1 alpha Antibody (34039)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-HIF1 alpha Mouse Monoclonal Antibody

SPECIFICITY SHORT :

HIF1 alpha

SPECIES REACTIVITY :

Human

TARGET NAME :

HIF1 alpha

UNIPROT :

Q61221

LOCATION :

Cytoplasm , Nucleus , Nucleus speckle

PROTEIN NAME :

Hypoxia-inducible factor 1-alpha

ALTERNATIVE NAMES :

HIF-1-alpha, HIF1-alpha, ARNT-interacting protein

GENE NAME :

Hif1a

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

289.1

SKU SIZE 1 :

34039

APPLICATIONS :

WB, IHC, ICC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 2ug/ml. Predicted molecular weight ~116kDa. This is a recommended concentration. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

ESEE122

IMMUNOGEN :

Recombinant protein corresponding to aa 329-530 of human HIF-1alpha.

RESEARCH AREA :

Cancer research

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor that regulates the transcription of a broad range of genes that facilitate responses to hypoxia including genes that regulate angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. HIF-1 is comprised of two subunits, HIF-1alpha and HIF-1beta. The widely expressed HIF-1alpha is degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH)-E3 ligase complex; ubiquitination and proteasomal degradation follows.

FUNCTION :

Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity). {UniProtKB:Q16665, PubMed:15225651, PubMed:17981124, PubMed:22009797, PubMed:26245371}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, rat, and bovine HIF-1alpha.

Image Name_1 :

qed-bioscience-anti-hif1-alpha-antibody-34039-1.png

Applications 1 :

IHC

Image Name_2 :

qed-bioscience-anti-hif1-alpha-antibody-34039-2.png

Applications 2 :

Western Blot