Anti-DOG1 Antibody | QED Bioscience 34036 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-DOG1 Antibody

catalog :

34036

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DOG1.1

The same clone is also sold as:

OriGene: AM33350PU-S, AM09094SU-S, AM33350PU-T, AM10078SU-N

reactivity :

human

product information

CATALOG NUMBER :

34036

PRODUCT NAME :

Anti-DOG1 Antibody

NAME+SKU :

Anti-DOG1 Antibody (34036)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-DOG1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

DOG1

SPECIES REACTIVITY :

Human

TARGET NAME :

DOG1

UNIPROT :

Q5XXA6

LOCATION :

Cell membrane , Cytoplasm

PROTEIN NAME :

Anoctamin-1

ALTERNATIVE NAMES :

Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2

GENE NAME :

ANO1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

289.1

SKU SIZE 1 :

34036

APPLICATIONS :

IHC

APPLICATION INSTRUCTIONS :

Immunohistochemistry: Use at 1- 10ug/ml on paraffin-embedded samples. Antigen retrieval solution: 10mM citrate, pH 6 (boil slides by microwaving in antigen retrieval solution for 12 minutes). User should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

DOG1.1

IMMUNOGEN :

Synthetic peptide corresponding to aa 904-986 of human DOG1 (accession no. Q5XXA6).

RESEARCH AREA :

Cancer research

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

DOG1 (Discovered on GIST1), also known as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as a gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a biomarker for GIST.

FUNCTION :

Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. {PubMed:20056604, PubMed:21984732, PubMed:22178883, PubMed:22946059}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human DOG1.

Image Name_1 :

qed-bioscience-anti-dog1-antibody-34036-1.jpg

Applications 1 :

IHC