Anti-Glypican-1 Antibody | QED Bioscience 34029 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Glypican-1 Antibody

catalog :

34029

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9-E9

The same clone is also sold as:

Santa Cruz Biotechnology: sc-101826

reactivity :

human, mouse

product information

CATALOG NUMBER :

34029

PRODUCT NAME :

Anti-Glypican-1 Antibody

NAME+SKU :

Anti-Glypican-1 Antibody (34029)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Glypican-1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Glypican-1

SPECIES REACTIVITY :

Human, mouse

TARGET NAME :

Glypican-1

UNIPROT :

P35052

LOCATION :

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains.; [Secreted glypican-1]: Secreted, extracellular space.

PROTEIN NAME :

Glypican-1 [Cleaved into: Secreted glypican-1]

GENE NAME :

GPC1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

289.1

SKU SIZE 1 :

34029

APPLICATIONS :

WB, IF

APPLICATION INSTRUCTIONS :

ELISA: at 1-5ug/ml will detect recombinant glypican-1 on the solid phase at 200ng/well. Immunoblotting: at 1-10ug/ml, a band of approx. 64 kDa is detected in normal mouse brain. Immunofluorescence: at 1-10ug/ml, positive staining of human pancreatic carcinoma cell line Capan-1. These are recommended concentrations. User should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

9-E9

IMMUNOGEN :

Recombinant full-length human glypican-1.

RESEARCH AREA :

Cancer research

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Glypican-1 is a widely expressed, GPI anchored, cell surface proteoglycan that has been reported to be important in cellular adhesion and migration. Glypican-1 also functions as an accessory molecule for signaling receptors. Glypican-1 is overexpressed in human gliomas and pancreatic ductal cell carcinomas. Expression of glypican-1 is associated with the aggressive behavior of tumor cells in vitro and in vivo.

FUNCTION :

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. {PubMed:19936054, PubMed:21642435}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable at 4C (short- term) and -70C (long-term).

SPECIFICITY :

This antibody recognizes native and recombinant human and mouse glypican-1.

Image Name_1 :

qed-bioscience-anti-glypican-1-antibody-34029-1.jpg

Applications 1 :

Image Name_2 :

qed-bioscience-anti-glypican-1-antibody-34029-2.jpg

Applications 2 :