Anti-Thymidylate synthase Antibody | QED Bioscience 34001 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Thymidylate synthase Antibody

catalog :

34001

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2D8.D11

reactivity :

human

product information

CATALOG NUMBER :

34001

PRODUCT NAME :

Anti-Thymidylate synthase Antibody

NAME+SKU :

Anti-Thymidylate synthase Antibody (34001)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Thymidylate Synthase Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Thymidylate Synthase

SPECIES REACTIVITY :

Human

TARGET NAME :

Thymidylate synthase

UNIPROT :

P04818

LOCATION :

Nucleus , Cytoplasm , Mitochondrion , Mitochondrion matrix , Mitochondrion inner membrane

PROTEIN NAME :

Thymidylate synthase

ALTERNATIVE NAMES :

TS, TSase, EC 2.1.1.45

GENE NAME :

TYMS

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

319.9

SKU SIZE 1 :

34001

APPLICATIONS :

WB, IP, IHC, ELISA

APPLICATION INSTRUCTIONS :

Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined). Immunoblotting: use at 5-10 ug/ml. ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml). Immunoprecipitation: use #34002 only at 5-10 ug/ml.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

2D8.D11

IMMUNOGEN :

Recombinant human thymidylate synthase produced in E. coli.

RESEARCH AREA :

Cancer research

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

FUNCTION :

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {PubMed:11278511, PubMed:21876188}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least one (1) year at -20C.

SPECIFICITY :

These antibodies specifically recognize human TS (36 kDa). They do not cross-react with rat TS.