Anti-TSG101 Antibody | QED Bioscience 3352 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-TSG101 Antibody

catalog :

3352

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4A10

The same clone is also sold as:

Invitrogen: MA1-23296
Abcam: ab83
GeneTex: GTX70255

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

3352

PRODUCT NAME :

Anti-TSG101 Antibody

NAME+SKU :

Anti-TSG101 Antibody (3352)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-TSG101 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

TSG101

SPECIES REACTIVITY :

Human, mouse, rat, hamster, and monkey

TARGET NAME :

TSG101

UNIPROT :

Q99816

LOCATION :

Cytoplasm , Early endosome membrane , Late endosome membrane , Midbody, Midbody ring , Nucleus

PROTEIN NAME :

Tumor susceptibility gene 101 protein

ALTERNATIVE NAMES :

ESCRT-I complex subunit TSG101

GENE NAME :

TSG101

ACCESSION NUMBER :

NP_006283

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ul

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

3352

APPLICATIONS :

ELSIA, WB, IHC

APPLICATION INSTRUCTIONS :

ELISA: use at 1-5ug/ml. Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500. Endusers should determine optimal antibody concentrations for their applications. IHC: use at a dilution of 1:100 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of TSG101 protein in paraffin-embedded human breast carcinoma. Detection of TSG101 protein in paraffin-embedded human ovarian carcinoma.

HOST :

Mouse

FORMAT :

Purified

ISOTYPE :

IgG1

CLONE ID :

4A10

IMMUNOGEN :

Recombinant protein corresponding to aa 167-374 of TSG101 protein

RESEARCH AREA :

Cancer Research

FORMULATION :

PBS, pH 7.2.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.2

FUNCTION :

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {PubMed:11916981, PubMed:17556548, PubMed:17853893, PubMed:21070952, PubMed:21757351, PubMed:22315426, PubMed:22660413}.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C.

SPECIFICITY :

This antibody recognizes human, mouse, rat, hamster, and monkey TSG101 protein.

Image Name_1 :

qed-bioscience-anti-tsg101-antibody-3352-1.jpg

Applications 1 :

Western Blot

Image Name_2 :

qed-bioscience-anti-tsg101-antibody-3352-2.jpg

Applications 2 :

ELISA

Image Name_3 :

qed-bioscience-anti-tsg101-antibody-3352-3.jpg

Applications 3 :

Image Name_4 :

qed-bioscience-anti-tsg101-antibody-3352-4.jpg

Image Caption_4 :

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications 4 :

IHC

Image Name_5 :

qed-bioscience-anti-tsg101-antibody-3352-5.jpg

Image Caption_5 :

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications 5 :

IHC

Image Name_6 :

qed-bioscience-anti-tsg101-antibody-3352-6.jpg

Image Caption_6 :

TSG101 antibody [4A10] (3352) detects TSG101 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: TSG101 antibody [4A10] (3352) dilution: 1:25. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Applications 6 :

Image Name_7 :

qed-bioscience-anti-tsg101-antibody-3352-7.jpg

Image Caption_7 :

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Applications 7 :

Image Name_8 :

qed-bioscience-anti-tsg101-antibody-3352-8.jpg

Image Caption_8 :

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications 8 :

Image Name_9 :

qed-bioscience-anti-tsg101-antibody-3352-9.jpg

Image Caption_9 :

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications 9 :

IHC

Image Name_10 :

qed-bioscience-anti-tsg101-antibody-3352-10.jpg

Image Caption_10 :

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications 10 :

Image Name_11 :

qed-bioscience-anti-tsg101-antibody-3352-11.jpg

Image Caption_11 :

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.