Anti-FKHR Antibody | QED Bioscience 3338 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-FKHR Antibody

catalog :

3338

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H3

The same clone is also sold as:

Novus Biologicals: NB200-307
Invitrogen: MA1-23236
OriGene: TA317832

reactivity :

human

product information

CATALOG NUMBER :

3338

PRODUCT NAME :

Anti-FKHR Antibody

NAME+SKU :

Anti-FKHR Antibody (3338)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-FKHR Mouse Monoclonal Antibody

SPECIFICITY SHORT :

FKHR

SPECIES REACTIVITY :

Human

TARGET NAME :

FKHR

UNIPROT :

Q12778

LOCATION :

Cytoplasm , Nucleus

PROTEIN NAME :

Forkhead box protein O1

ALTERNATIVE NAMES :

Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

GENE NAME :

FOXO1

ACCESSION NUMBER :

NP_002006

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

3338

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.1-2.0ug/ml. A band of 70kDa is detected. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

7H3

IMMUNOGEN :

GST fusion protein containing the region encoding aa 242-655 of human FKHR expressed in E. coli.

RESEARCH AREA :

Cancer Research

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

FKHR (ForKHead in Rhabdomyosarcoma), a 70kDa protein, is a member of the forkhead family of transcription factors. Members of this family are classified by their degree of homology in the 100-120aa region that is necessary for DNA binding and binding-site specificity. The FKHR subfamily differs from other members of the forkhead family in that all members contain an insertion of 5 amino acids next to the helix 3 in the middle of the forkhead domain and lack the conserved amino terminal KPPY sequence found in most forkhead genes. It has been shown that members of the FKHR gene family are phosphorylated by Akt which regulates its cellular localization promoting cell survival.

FUNCTION :

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed:10358076). Activity suppressed by insulin (PubMed:10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (By similarity). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed:18356527, PubMed:19221179). Promotes neural cell death (PubMed:18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed:20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed:31063815). {UniProtKB:A4L7N3, UniProtKB:G3V7R4, UniProtKB:Q9R1E0, PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099, PubMed:31063815}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human FKHR/FOX01.

Image Name_1 :

qed-bioscience-anti-fkhr-antibody-3338-1.jpg

Applications 1 :

IHC

Image Name_2 :

qed-bioscience-anti-fkhr-antibody-3338-2.jpg

Applications 2 :