Anti-Estrogen Receptor alpha Antibody | QED Bioscience 3335 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Estrogen Receptor alpha Antibody

catalog :

3335

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F3

reactivity :

human

product information

CATALOG NUMBER :

3335

PRODUCT NAME :

Anti-Estrogen Receptor alpha Antibody

NAME+SKU :

Anti-Estrogen Receptor alpha Antibody (3335)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Estrogen Receptor Alpha Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Estrogen Receptor Alpha

SPECIES REACTIVITY :

Human

TARGET NAME :

Estrogen Receptor alpha

UNIPROT :

P03372

LOCATION :

[Isoform 1]: Nucleus , Cytoplasm , Cell membrane

PROTEIN NAME :

Estrogen receptor

ALTERNATIVE NAMES :

ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

GENE NAME :

ESR1

ACCESSION NUMBER :

NP_000116

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

3335

APPLICATIONS :

WB, IP

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. Immunohistochemistry: frozen sections, use at 1-10ug/ml. FACS: use at 1-10ug/ml. Immunoprecipitation: use at 1-5ug/ml. Positive controls: MCF-7 cells and recombinant protein. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

1F3

IMMUNOGEN :

6-His fusion protein containing the region encoding aa 1-190 of human estrogen receptor-alpha (ER-alpha) expressed in E. coli.

RESEARCH AREA :

Cancer Research

FORMULATION :

PBS, pH 7.4, 1mg/ml.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). {PubMed:10681512, PubMed:10816575, PubMed:11477071, PubMed:11682626, PubMed:14764652, PubMed:15078875, PubMed:15891768, PubMed:16043358, PubMed:16617102, PubMed:16684779, PubMed:17922032, PubMed:17932106, PubMed:18247370, PubMed:19350539, PubMed:20074560, PubMed:20705611, PubMed:21330404, PubMed:22083956, PubMed:7651415, PubMed:9328340}.; [Isoform 3]: Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861). {PubMed:10970861, PubMed:21937726}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human and rabbit ER-alpha, a 65 kD protein and a member of the steroid family of nuclear receptors. ER-alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, ER-alpha can positively or negatively regulate gene transcription.

Image Name_1 :

qed-bioscience-anti-estrogen-receptor-alpha-antibody-3335-1.jpg

Applications 1 :

Western Blot

Image Name_2 :

qed-bioscience-anti-estrogen-receptor-alpha-antibody-3335-2.jpg

Applications 2 :

Image Name_3 :

qed-bioscience-anti-estrogen-receptor-alpha-antibody-3335-3.jpg

Applications 3 :