Anti-ATM Antibody | QED Bioscience 3310 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-ATM Antibody

catalog :

3310

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2C1

The same clone is also sold as:

GeneTex: GTX70103
Novus Biologicals: NB100-309
Invitrogen: MA1-23152, MA5-31599

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

3310

PRODUCT NAME :

Anti-ATM Antibody

NAME+SKU :

Anti-ATM Antibody (3310)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-ATM Mouse Monoclonal Antibody

SPECIFICITY SHORT :

ATM

SPECIES REACTIVITY :

Human, mouse, rat, monkey

TARGET NAME :

ATM

UNIPROT :

Q13315

LOCATION :

Nucleus , Cytoplasmic vesicle , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

PROTEIN NAME :

Serine-protein kinase ATM

ALTERNATIVE NAMES :

EC 2.7.11.1, Ataxia telangiectasia mutated, A-T mutated

GENE NAME :

ATM

ACCESSION NUMBER :

NP_000042

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

3310

APPLICATIONS :

WB, IP

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10 ug/ml. Immunoprecipitation: use at 1-10 ug/ml. Positive controls: Raji or Akata cells.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

2C1

IMMUNOGEN :

GST fusion protein expressed in E. coli corresponding to aa 2577- 3056 of full-length ATM.

RESEARCH AREA :

Cancer Research

FORMULATION :

PBS, pH 7.4

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. RPubMed:10550055, PubMed:10766245, PubMed:10802669, PubMed:10839545, PubMed:10910365, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:12556884, PubMed:14871926, PubMed:15448695, PubMed:15456891, PubMed:15916964, PubMed:16086026, PubMed:16858402, PubMed:17923702, PubMed:19431188, PubMed:19965871, PubMed:26774286, PubMed:29203878, PubMed:30612738, PubMed:30886146, PubMed:9733514, PubMed:9733515, PubMed:9843217}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes full-length ATM, a 370 kD nuclear phosphoprotein, which is involved in the autooal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigations.

Image Name_1 :

qed-bioscience-anti-atm-antibody-3310-1.jpg

Applications 1 :

IHC

Image Name_2 :

qed-bioscience-anti-atm-antibody-3310-2.jpg

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-atm-antibody-3310-3.jpg

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-atm-antibody-3310-4.jpg

Image Caption_4 :

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (3310) diluted at 1:1000.

Applications 4 :

Image Name_5 :

qed-bioscience-anti-atm-antibody-3310-5.jpg

Image Caption_5 :

HeLa whole cell extract and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (3310) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications 5 :

Image Name_6 :

qed-bioscience-anti-atm-antibody-3310-6.jpg

Image Caption_6 :

ATM antibody [2C1] detects ATM protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ATM stained by ATM antibody [2C1] (3310) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications 6 :

IHC

Image Name_7 :

qed-bioscience-anti-atm-antibody-3310-7.jpg

Image Caption_7 :

Untreated (–) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (3310) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications 7 :