Anti-Rb Protein Antibody | QED Bioscience 3104 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Rb Protein Antibody

catalog :

3104

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1G5

reactivity :

human

product information

CATALOG NUMBER :

3104

PRODUCT NAME :

Anti-Rb Protein Antibody

NAME+SKU :

Anti-Rb Protein Antibody (3104)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Rb Protein Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Rb Protein

ASSOCIATED PRODUCTS :

Associated Clone Specificity Notes Catalog No. Clone No. Amino Acid Specificity ELISA Western Blot Immuno-Precipitation Immuno-Histochemistry . 3101 . 4G2 . 41-330 . . . . . 3102 . 4H6 . 121-140 . . . . . 3103 . 7F12 . 280-300 . . . . - . 3104 . 1G5 . 371-390 . . . . . 3105 . 1E5 . 511-530 . . . . - . 3106 . 1D1 . 571-590 . . . . - . 3107 . 3C8 . 886-905 . . . .

SPECIES REACTIVITY :

Human

TARGET NAME :

Rb Protein

UNIPROT :

P06400

LOCATION :

Nucleus

PROTEIN NAME :

Retinoblastoma-associated protein

ALTERNATIVE NAMES :

p105-Rb, p110-RB1, pRb, Rb, pp110

GENE NAME :

RB1

ACCESSION NUMBER :

NP_000312

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

246.4

SKU SIZE 1 :

3104

APPLICATIONS :

WB, IP, IHC, ELISA

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

1G5

IMMUNOGEN :

Recombinant retinoblastoma

RESEARCH AREA :

Cancer Research

FORMULATION :

PBS, pH 7.4

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}.; (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least protein (p110RB) produced in E. coli. o o Host Species: Mouse Antibody Class: 3101-3106 = IgG1; 3107 = IgG2a. Stabilizers: None Preservatives: None. Available on request.

SPECIFICITY :

Recombinant retinoblastoma

Image Name_1 :

qed-bioscience-anti-rb-protein-antibody-3104-1.jpg