Anti-Fibroblast Growth Factor Receptor 1 alpha (FGFR1alpha) Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Fibroblast Growth Factor Receptor 1 alpha (FGFR1alpha) Antibody

catalog :

30104

quantity :

250 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M2F12

The same clone is also sold as:

Santa Cruz Biotechnology: sc-57132 AC, sc-57132 HRP, sc-57132
Novus Biologicals: NB120-829, NBP2-80734
Invitrogen: 37-9200, MA1-26256

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

30104

PRODUCT NAME :

Anti-Fibroblast Growth Factor Receptor 1 alpha (FGFR1alpha) Antibody

NAME+SKU :

Anti-Fibroblast Growth Factor Receptor 1 alpha (FGFR1alpha) Antibody (30104)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-FGF Receptor-alpha Mouse Monoclonal Antibody

SPECIFICITY SHORT :

FGF Receptor-alpha

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Fibroblast Growth Factor Receptor 1 alpha (FGFR1alpha)

UNIPROT :

P11362

LOCATION :

Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus.

PROTEIN NAME :

Fibroblast growth factor receptor 1

ALTERNATIVE NAMES :

FGFR-1, EC 2.7.10.1, Basic fibroblast growth factor receptor 1, BFGFR, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, N-sam, Proto-oncogene c-Fgr, CD antigen CD331

GENE NAME :

FGFR1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

250 ug

DISTIRBUTOR PRICE :

417.2

SKU SIZE 1 :

30104

APPLICATIONS :

WB, IHC, IP, FACS

APPLICATION INSTRUCTIONS :

Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: frozen sections of human glioblastomas, para-formaldehyde- fixed cells and nuclei, human myocardial biopsies.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

M2F12

IMMUNOGEN :

Recombinant human ectodomain of FGFr1alpha expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH- terminal glu-phe sequences.

RESEARCH AREA :

Growth Factors, Cytokines, Receptors

FORMULATION :

PBS, pH 7.4

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. {UniProtKB:P16092, PubMed:10830168, PubMed:11353842, PubMed:12181353, PubMed:1379697, PubMed:1379698, PubMed:15117958, PubMed:16597617, PubMed:17311277, PubMed:17623664, PubMed:18480409, PubMed:19224897, PubMed:19261810, PubMed:19665973, PubMed:20133753, PubMed:20139426, PubMed:21765395, PubMed:8622701, PubMed:8663044}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

30104 reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1alpha. Reacts with human, rat, and mouse receptor. Other species untested.