Anti-Tumor Necrosis Factor Alpha Antibody | QED Bioscience 21303 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Tumor Necrosis Factor Alpha Antibody

catalog :

21303

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F6C5

The same clone is also sold as:

OriGene: 4T10-F6C5
Invitrogen: MA1-26970
GeneTex: GTX28349

reactivity :

human

product information

CATALOG NUMBER :

21303

PRODUCT NAME :

Anti-Tumor Necrosis Factor Alpha Antibody

NAME+SKU :

Anti-Tumor Necrosis Factor Alpha Antibody (21303)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Tumor Necrosis Factor Alpha Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Tumor Necrosis Factor Alpha

SPECIES REACTIVITY :

Human

TARGET NAME :

Tumor Necrosis Factor Alpha

UNIPROT :

P01375

LOCATION :

Cell membrane , Single-pass type II membrane protein

PROTEIN NAME :

Tumor necrosis factor

ALTERNATIVE NAMES :

Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a [Cleaved into: Tumor necrosis factor, membrane form

GENE NAME :

TNF

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

213.5

SKU SIZE 1 :

21303

APPLICATIONS :

ELISA, IHC

APPLICATION INSTRUCTIONS :

These antibodies may be used in immunoassays or in immunohisto- chemistry to detect and/or quantitate TNFalpha. 21301 and 21302 may be used in biological assays to inhibit activity of TNFalpha. Other applications are under investigation.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

F6C5

IMMUNOGEN :

Recombinant human TNFalpha.

RESEARCH AREA :

Growth Factors, Cytokines, Receptors

FORMULATION :

PBS containing 0.1% sodium azide

BUFFER :

Phosphate Buffered Saline

BUFFER ANTI-MICROBIAL :

0.1% Sodium Azide

FUNCTION :

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). {UniProtKB:P06804, PubMed:12794819, PubMed:16829952, PubMed:22517918, PubMed:23396208}.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {PubMed:16829952}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

21303 recognizes a different epitope on TNFalpha than 21301 and 21302.21301 and 21302 can inhibit the biological activity of recombinant and native TNFalpha.