antibody

Anti-Lactoferrin Antibody

21101

100 ug

monoclonal

mouse

nonconjugated

2B8

The same clone is also sold as:

• OriGene: 4L2-2B8
• Bio-Rad: MCA2763

Anti-Lactoferrin Antibody

Anti-Lactoferrin Antibody (21101)

Antibody

Anti-Lactoferrin Mouse Monoclonal Antibody

Lactoferrin

Human

Lactoferrin

P02788

[Isoform 1]: Secreted. Cytoplasmic granule. Note=Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils.; [Isoform DeltaLf]: Cytoplasm. Nucleus. Note=Mainly localized in the cytoplasm.

Lactotransferrin

Lactoferrin, EC 3.4.21.-, Growth-inhibiting protein 12, Talalactoferrin [Cleaved into: Lactoferricin-H

LTF

Monoclonal

Liquid

Lot Specific

100 ug

159.6

21101

WB, ELISA

This antibody may be used in immuno-assays to detect and quantitate human lactoferrin. Not all applications have been investigated.

Mouse

Purified

Purified by immunoaffinity chromatography

IgG1

2B8

Purified lactoferrin from human milk.

Hormones/Proteins

PBS containing 0.1% sodium azide

Phosphate Buffered Saline

0.1% Sodium Azide

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. {PubMed:22900286}.; [Lactotransferrin]: Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969). Has antimicrobial activity, which depends on the extracellular cation concentration (PubMed:6802759). Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969). Can also prevent bacterial biofilm development in P.aeruginosa infection (PubMed:12037568). Has weak antifungal activity against C.albicans (PubMed:11083624). Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth (PubMed:15166119). Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection (PubMed:17079302). Can inhibit papillomavirus infections (PubMed:17481742). Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling (PubMed:20345905). Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells (PubMed:19033648). Stimulates VEGFA-mediated endothelial cell migration and proliferation (PubMed:16842782). Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs) (PubMed:9359845). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA (PubMed:9359845). {PubMed:11083624, PubMed:11179314, PubMed:12037568, PubMed:12693969, PubMed:14573629, PubMed:15166119, PubMed:1599934, PubMed:16842782, PubMed:17079302, PubMed:17481742, PubMed:19033648, PubMed:20345905, PubMed:3169987, PubMed:6802759, PubMed:9359845}.; Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection (PubMed:17481742). N-terminal region shows strong antifungal activity against C.albicans (PubMed:11083624). Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. {PubMed:11083624, PubMed:17481742}.; [Kaliocin-1]: Has antimicrobial activity and is able to permeabilize different ions through liposomal membranes. {PubMed:12693969}.; [Lactoferroxin-A]: Has opioid antagonist activity (PubMed:1369293). Shows preference for mu-receptor (PubMed:1369293). {PubMed:1369293}.; [Lactoferroxin-B]: Has opioid antagonist activity (PubMed:1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed:1369293). {PubMed:1369293}.; [Lactoferroxin-C]: Has opioid antagonist activity (PubMed:1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed:1369293). {PubMed:1369293}.; The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions (PubMed:12535064). This function contributes to the antimicrobial activity (PubMed:12535064). Shows a preferential cleavage at -Arg-Ser-Arg-Arg- - and -Arg-Arg-Ser-Arg- -, and of Z-Phe-Arg- -aminomethylcoumarin sites (PubMed:12535064). {PubMed:12535064}.; [Isoform DeltaLf]: Transcription factor with antiproliferative properties and ability to induce cell cycle arrest (PubMed:15222485). Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters (PubMed:22320386). {PubMed:15222485, PubMed:22320386}.

Dilute in PBS or medium that is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C to -70C. Store antibody in appropriate aliquots to avoid multiple freeze-thaw cycles.

This antibody reacts in Western blots of whole serum proteins with a lactoferrin band of 77kD. No cross-reactivity with other human serum proteins.