Anti-High MW Kininogen Light Chain Antibody | QED Bioscience 20001 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-High MW Kininogen Light Chain Antibody

catalog :

20001

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C11C1

The same clone is also sold as:

Invitrogen: MA1-10831
Santa Cruz Biotechnology: sc-23915 AC, sc-23915, sc-23915 HRP

reactivity :

human

product information

CATALOG NUMBER :

20001

PRODUCT NAME :

Anti-High MW Kininogen Light Chain Antibody

NAME+SKU :

Anti-High MW Kininogen Light Chain Antibody (20001)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-High MW Kininogen - Light Chain Mouse Monoclonal Antibody

SPECIFICITY SHORT :

High MW Kininogen - Light Chain

SPECIES REACTIVITY :

Human

TARGET NAME :

High MW Kininogen Light Chain

UNIPROT :

P01042

LOCATION :

Secreted, extracellular space.

PROTEIN NAME :

Kininogen-1

ALTERNATIVE NAMES :

Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor [Cleaved into: Kininogen-1 heavy chain; T-kinin

GENE NAME :

KNG1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

415.8

SKU SIZE 1 :

20001

APPLICATIONS :

WB, ELISA, coagulation assays

APPLICATION INSTRUCTIONS :

This antibody may be used in ELISA and Western blots to detect human HMWK light chain. It may also be used in coagulation assays. Enduser should determine optimal antibody concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

C11C1

IMMUNOGEN :

Purified human high molecular weight kininogen.

RESEARCH AREA :

Coagulation factors

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

High molecular weight kininogen (HMWK) is a circulating plasma protein which participates in the initiation of blood coagulation, and in the generation of the vasodilator bradykinin via the Kallikrein-kinin system. HMWK is inactive until it either adheres to binding proteins beneath an endothelium disrupted by injury, thereby initiating coagulation, or it binds to intact endothelial cells or platelets for functions other than coagulation. HMWK is an alpha-globulin with six functional domains. It circulates as a single-chain 626 amino acid polypeptide . The heavy chain contains domains 1, 2, and 3; the light chain, domains 5 and 6. Domain 4 links the heavy and light chains.

FUNCTION :

(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes an epitope on the unique 46kDa light chain of human HMWK. The epitope maps to domain 5 of human HMWK. It can completely neutralize the coagulant activity of human HMWK.

Image Name_1 :

qed-bioscience-anti-high-mw-kininogen-light-chain-antibody-20001-1.jpg

Applications 1 :