Anti-Prekallikrein Heavy Chain Antibody | QED Bioscience 19901 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Prekallikrein Heavy Chain Antibody

catalog :

19901

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13G11

The same clone is also sold as:

Abcam: ab1006
Invitrogen: MA1-10830, MA1-26321

reactivity :

human

product information

CATALOG NUMBER :

19901

PRODUCT NAME :

Anti-Prekallikrein Heavy Chain Antibody

NAME+SKU :

Anti-Prekallikrein Heavy Chain Antibody (19901)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Prekallikrein Heavy Chain Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Prekallikrein Heavy Chain

SPECIES REACTIVITY :

Human

TARGET NAME :

Prekallikrein Heavy Chain

UNIPROT :

P03952

LOCATION :

Secreted.

PROTEIN NAME :

Plasma kallikrein

ALTERNATIVE NAMES :

EC 3.4.21.34, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain]

GENE NAME :

KLKB1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

415.8

SKU SIZE 1 :

19901

APPLICATIONS :

WB, ELISA

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. Bands of 85kDa and 88kDa are detected. ELISA: use at 5-10ug/ml with prekallikrein sample bound to ELISA plates. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

13G11

IMMUNOGEN :

Purified human prekallikrein.

RESEARCH AREA :

Coagulation factors

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Human prekallikrein, a serine protease zymogen, is present in plasma as two variants, 88kDa and 85kDa, probably due to different carbohydrate content. Approximately 75% of the total prekallikrein in plasma is noncovalently associated with high molecular weight kininogen (HMWK). HMWK mediates binding of prekallikrein to a negatively-charged surface which is required for its optimal activation to kallikrein by activated factor XII. Although kallikrein light and heavy chains can behave as functionally independent domains, the interaction between these two chains is essential for full biologic activity.

FUNCTION :

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This product does not contain preservative. The stock solution (2.0mg/ml) can be stored in aliquots at â€“ 20C for 1 year or can be stored at 4C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored. Avoid repeated freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes two variants of prekallikrein in human plasma, 88kDa and 85kDa, and its activation products kallikrein, the complexes formed by kallikrein with its endogenous inhibitors C1 inhibitor, alpha-2-macroglobulin and antithrombin III, and 45kDa prekallikrein/kallikrein fragments. It also recognizes prekallikrein and its activation products in chimpanzee, rhesus, and baboon plasmas. The epitope for this antibody is located on the prekallikrein/kallikrein hevy chain and is involved in the interaction between prekallikrein and factor XIIa. This antibody inhibits prekallikrein activation in human and rhesus plasmas by ~60- 80% and 55%, respectively. This antibody does not cross-react with tissue kallikrein.

Image Name_1 :

qed-bioscience-anti-prekallikrein-heavy-chain-antibody-19901-1.jpg

Applications 1 :