Anti-CD4 Cytoplasmic Tail Antibody | QED Bioscience 18714 product information

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product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-CD4 Cytoplasmic Tail Antibody

catalog :

18714

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8A5

reactivity :

human

product information

CATALOG NUMBER :

18714

PRODUCT NAME :

Anti-CD4 Cytoplasmic Tail Antibody

NAME+SKU :

Anti-CD4 Cytoplasmic Tail Antibody (18714)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-CD4 Cytoplasmic Tail Mouse Monoclonal Antibody

SPECIFICITY SHORT :

CD4 Cytoplasmic Tail

SPECIES REACTIVITY :

Human

TARGET NAME :

CD4 Cytoplasmic Tail

UNIPROT :

P01730

LOCATION :

Cell membrane

PROTEIN NAME :

T-cell surface glycoprotein CD4

ALTERNATIVE NAMES :

T-cell surface antigen T4/Leu-3, CD antigen CD4

GENE NAME :

CD4

ACCESSION NUMBER :

NP_000607

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

250.6

SKU SIZE 1 :

18714

APPLICATIONS :

ELISA, IF

APPLICATION INSTRUCTIONS :

ELISA: use at 10-20ug/ml. Optimized with 25ug cell lysate (Jurkat, H9, HeLa) on the solid phase. Immunofluorescence: use at 5-10ug/ml. Optimized with CEM cells adhered to glass slides with poly-L-lysine and fixed in paraformaldehyde.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgM

CLONE ID :

8A5

IMMUNOGEN :

Synthetic peptide corresponding to aa sequence RFLPADQMNVADPQPLASCS at the post 3â€™-UGA codon region of CD4.

RESEARCH AREA :

Cell adhesion

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

CD4 is a co-receptor of the T-cell receptor (TCR) and assists the TCR in communicating with antigen-presenting cells. The short cytoplasmic/intracellular tail of CD4 contains a special sequence of amino acids that allow it to recruit and interact with the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase). When the TCR and CD4 bind to distinct regions of an antigen-presenting MHCII molecule, tyrosine kinase Lck bound to the cytoplasmic tail of CD4 tyrosine-phosphorylates immunoreceptor tyrosine activation motifs (ITAM) on the cytoplasmic domains of CD3 to amplify the signal generated by the TCR. Many functions have been assigned to the cytoplasmic tail of CD4 including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transmission.

FUNCTION :

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. {PubMed:16951326, PubMed:24942581, PubMed:2823150, PubMed:7604010}.; (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607). {PubMed:12089508, PubMed:16331979, PubMed:17346169, PubMed:2214026, PubMed:7909607, PubMed:9641677}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C to -70C. Store antibody in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes the post 3â€™-UGA codon region of human CD4.