Anti-DNA Polymerase delta Antibody | QED Bioscience 13910 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-DNA Polymerase delta Antibody

catalog :

13910

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6196

reactivity :

human

product information

CATALOG NUMBER :

13910

PRODUCT NAME :

Anti-DNA Polymerase delta Antibody

NAME+SKU :

Anti-DNA Polymerase delta Antibody (13910)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-DNA Polymerase delta Mouse Monoclonal Antibody

SPECIFICITY SHORT :

DNA Polymerase delta

SPECIES REACTIVITY :

Human

TARGET NAME :

DNA Polymerase delta

UNIPROT :

P28340

LOCATION :

Nucleus

PROTEIN NAME :

DNA polymerase delta catalytic subunit

ALTERNATIVE NAMES :

EC 2.7.7.7, 3'-5' exodeoxyribonuclease, EC 3.1.11.-, DNA polymerase subunit delta p125

GENE NAME :

POLD1

ACCESSION NUMBER :

NP_001243778.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

322.7

SKU SIZE 1 :

13910

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at a 1:500-1:3,000 dilution. A band of ~124kDa is detected. Detection of DNA polymerase delta in HeLa cell lysate with #13910 diluted 1:1,000. IHC: use at a dilution of 1:200 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of DNA polymerase delta in paraffin-embedded human colon carcinoma. Endusers should determine optimal antibody concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

ISOTYPE :

IgG1

CLONE ID :

6196

IMMUNOGEN :

Recombinant protein corresponding to aa 1-140 of the large subunit of human DNA polymerase delta

RESEARCH AREA :

Enzymes

FORMULATION :

PBS, pH 7.2.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.2

FUNCTION :

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025). {PubMed:16510448, PubMed:19074196, PubMed:20227374, PubMed:20334433, PubMed:24022480, PubMed:24035200, PubMed:24191025, PubMed:24310611}.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human DNA polymerase delta.

Image Name_1 :

qed-bioscience-anti-dna-polymerase-delta-antibody-13910-1.jpg

Applications 1 :

IHC

Image Name_2 :

qed-bioscience-anti-dna-polymerase-delta-antibody-13910-2.jpg

Applications 2 :