Anti-Heme Oxygenase-1 Antibody | QED Bioscience 13061 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Heme Oxygenase-1 Antibody

catalog :

13061

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F12.A6

The same clone is also sold as:

StressMarq Biosciences: SMC-131
OriGene: TA326354
Abcam: ab223349
Invitrogen: MA5-45152, MA5-45153, MA5-45154

reactivity :

human, mouse, bovine

product information

CATALOG NUMBER :

13061

PRODUCT NAME :

Anti-Heme Oxygenase-1 Antibody

NAME+SKU :

Anti-Heme Oxygenase-1 Antibody (13061)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Heme Oxygenase-1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Heme Oxygenase-1

SPECIES REACTIVITY :

Human, mouse, bovine

TARGET NAME :

Heme Oxygenase-1

UNIPROT :

P09601

LOCATION :

Microsome , Endoplasmic reticulum membrane , Peripheral membrane protein , Cytoplasmic side

PROTEIN NAME :

Heme oxygenase 1

ALTERNATIVE NAMES :

HO-1, EC 1.14.14.18

GENE NAME :

HMOX1

ACCESSION NUMBER :

NP_002124.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

242.9

SKU SIZE 1 :

13061

APPLICATIONS :

WB, ELISA

APPLICATION INSTRUCTIONS :

ELISA: use at 1ug/ml with HO-1 on the solid phase. Immunoblotting: use at 0.5-1ug/ml. A band of ~32kDa is detected. Immunofluorescence: use at 5-10ug/ml Positive control: Human cell line lysates such as A431, A549, HeLa, HepG2, Jurkat, or MCF7. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

1F12.A6

IMMUNOGEN :

Synthetic peptide corresponding to aa 1-30 of human HO-1.

RESEARCH AREA :

Enzymes

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.

FUNCTION :

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

Stock solution of 2mg/ml should be stored for one (1) year at -20C in appropriate aliquots to avoid multiple freeze-thaw cycles or at 4C for 6 months with the addition of 0.1% sodium azide. Diluted antibody should not be stored.

SPECIFICITY :

This antibody recognizes human, mouse, and bovine HO-1. It does not cross-react with HO-2.

Image Name_1 :

qed-bioscience-anti-heme-oxygenase-1-antibody-13061-1.jpg

Applications 1 :

Image Name_2 :

qed-bioscience-anti-heme-oxygenase-1-antibody-13061-2.jpg

Applications 2 :