Anti-CENP-A Antibody | QED Bioscience 12597 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-CENP-A Antibody

catalog :

12597

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A7-2E11

The same clone is also sold as:

OriGene: TA326348
Invitrogen: MA5-27710, MA5-45297, MA5-45299, MA5-45298, MA5-45300
StressMarq Biosciences: SMC-202

reactivity :

human

product information

CATALOG NUMBER :

12597

PRODUCT NAME :

Anti-CENP-A Antibody

NAME+SKU :

Anti-CENP-A Antibody (12597)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-CENP-A Mouse Monoclonal Antibody

SPECIFICITY SHORT :

CENP-A

SPECIES REACTIVITY :

Human

TARGET NAME :

CENP-A

UNIPROT :

P49450

LOCATION :

Nucleus , Chromosome, centromere, kinetochore , Chromosome, centromere

PROTEIN NAME :

Histone H3-like centromeric protein A

ALTERNATIVE NAMES :

Centromere autoantigen A, Centromere protein A, CENP-A

GENE NAME :

CENPA

ACCESSION NUMBER :

NP_001035891.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

242.9

SKU SIZE 1 :

12597

APPLICATIONS :

WB, ELISA

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. A band of ~18kDa is detected. Detection of CENP-A in lysate of U2OS cells ELISA: use at 1-10ug/ml with CENP-A on the solid phase. Positive control: U2OS cell lysate These are recommended concentrations; enduser should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

5A7-2E11

IMMUNOGEN :

Synthetic peptide corresponding to a sequence within human CENP-A.

RESEARCH AREA :

DNA

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Replicated chromosomes include two kinetochores that control chromosome segregation during mitosis. Centromere Protein-A (CENP-A), a histone H3-like protein, contains a C-terminal H3-like domain which is required for centromere localization of CENP-A. It is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H2A, H2B, and H4. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on chromosomes.

FUNCTION :

Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292). {PubMed:11756469, PubMed:14667408, PubMed:15282608, PubMed:15475964, PubMed:15702419, PubMed:17651496, PubMed:18072184, PubMed:19114591, PubMed:21478274, PubMed:23818633, PubMed:25556658, PubMed:26878239, PubMed:27499292, PubMed:7962047, PubMed:9024683, PubMed:20739937}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human CENP-A. Reactivity with other species has not been investigated.

Image Name_1 :

qed-bioscience-anti-cenp-a-antibody-12597-1.jpg

Applications 1 :