This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-MDC1 Antibody
catalog :
12596
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P2B11

The same clone is also sold as:
reactivity :
human, mouse, bovine, chimpanzee
product information
CATALOG NUMBER :
12596
PRODUCT NAME :
Anti-MDC1 Antibody
NAME+SKU :
Anti-MDC1 Antibody (12596)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-MDC1 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
MDC1
SPECIES REACTIVITY :
Human, mouse, bovine, chimpanzee
TARGET NAME :
MDC1
UNIPROT :
Q5PSV9
LOCATION :
Nucleus. Chromosome. Note=Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks (By similarity).
PROTEIN NAME :
Mediator of DNA damage checkpoint protein 1
GENE NAME :
Mdc1
ACCESSION NUMBER :
NP_001010833.2
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
1.0 mg/mL
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
298.9
SKU SIZE 1 :
12596
APPLICATIONS :
WB, ICC
APPLICATION INSTRUCTIONS :
Immunocytochemistry Immunoblotting: use at 1ug/ml. A band of ~184kDa is detected. Note: There are 4 isoforms of human MDC1 with molecular weights of approximately 227, 198, 196, and 117kDa. (For more information see Lou et al. 2003, Nature 421: 957-961) These are recommended concentrations; enduser should determine optimal concentrations for their applications. Positive control: HeLa cell lysate.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG1
CLONE ID :
P2B11
IMMUNOGEN :
GST-tagged recombinant mouse MDC1 (accession no. NP_001010833.2).
RESEARCH AREA :
DNA
FORMULATION :
PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
BUFFER ANTI-MICROBIAL :
0.09% Sodium Azide
BUFFER CRYOPRESERVATIVE :
50% Glycerol
TARGET BACKGROUND :
Recent studies have shown that MDC1 (mediator of DNA damage checkpoint protein1) regulates many aspects of DNA damage response pathways, such as intra-S phase checkpoint, G2/M checkpoint, and radiation-induced apoptosis. Many proteins, such as ATM, BRCA1, and Chk2, interact with MDC1. MDC1 contains several protein-protein interaction domains. MDC1 appears to function as an adaptor protein, recruiting downstream proteins to upstream kinases and facilitating signal transduction following DNA damage.
FUNCTION :
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (By similarity). {ECO:0000250}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze- thaw cycles.
SPECIFICITY :
This antibody recognizes human, mouse, bovine and chimpanzee MDC1. Specific epitope is at the N-terminus of MDC1.
Image Name_1 :
qed-bioscience-anti-mdc1-antibody-12596-1.png
Applications 1 :
ICC
Image Name_2 :
qed-bioscience-anti-mdc1-antibody-12596-2.png
Applications 2 :
Western Blot
Image Name_3 :
qed-bioscience-anti-mdc1-antibody-12596-3.png
Applications 3 :
Western Blot
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States