Anti-DNMT1 Antibody | QED Bioscience 12595 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-DNMT1 Antibody

catalog :

12595

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G11-C7

The same clone is also sold as:

StressMarq Biosciences: SMC-201
Invitrogen: MA5-45294, MA5-45295, MA5-45296
OriGene: TA326440

reactivity :

human, mouse, zebrafish

product information

CATALOG NUMBER :

12595

PRODUCT NAME :

Anti-DNMT1 Antibody

NAME+SKU :

Anti-DNMT1 Antibody (12595)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-DNMT1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

DNMT1

SPECIES REACTIVITY :

Human, mouse, Zebrafish

TARGET NAME :

DNMT1

UNIPROT :

P26358

LOCATION :

Nucleus

PROTEIN NAME :

DNA

ALTERNATIVE NAMES :

cytosine-5-methyltransferase 1

GENE NAME :

DNMT1

ACCESSION NUMBER :

NP_001370

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

242.9

SKU SIZE 1 :

12595

APPLICATIONS :

WB, ELISA

APPLICATION INSTRUCTIONS :

ELISA Immunoblotting: use at 2ug/ml. A band of ~190kDa is detected. These are recommended concentrations; enduser should determine optimal concentrations for their applications. Positive controls: ES and H1299 cell lysates.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2b

CLONE ID :

4G11-C7

IMMUNOGEN :

Synthetic peptide corresponding to aa 637-650 of human DNMT1 (accession no. NP_001370).

RESEARCH AREA :

DNA

FORMULATION :

PBS, pH 7.4, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

TARGET BACKGROUND :

Methylation of DNA at cytosine residues is important in regulation of gene expression and genomic imprinting. Hyper- methylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked to development of cancer. Thus far three families of mammalian DNA methyl- transferase genes have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence- specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.

FUNCTION :

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306). {PubMed:16357870, PubMed:18413740, PubMed:18754681, PubMed:24623306}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term use.

SPECIFICITY :

This antibody recognizes human, mouse, and Zebrafish DNMT1.