Anti-Dicer Antibody | QED Bioscience 12532 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Dicer Antibody

catalog :

12532

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S167-7

The same clone is also sold as:

OriGene: TA326471

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

12532

PRODUCT NAME :

Anti-Dicer Antibody

NAME+SKU :

Anti-Dicer Antibody (12532)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Dicer Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Dicer

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Dicer

UNIPROT :

Q8R418

LOCATION :

Cytoplasm

PROTEIN NAME :

Endoribonuclease Dicer

ALTERNATIVE NAMES :

EC 3.1.26.3, Double-strand-specific ribonuclease mDCR-1

GENE NAME :

Dicer1

ACCESSION NUMBER :

NP_683750.2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

12532

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. Predicted molecular weight is ~215kDa. Positive Control: rat brain lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S167-7

IMMUNOGEN :

Fusion protein corresponding to aa 1638-1899 of mouse Endoribonuclease Dicer (accession no.Q8R418).

RESEARCH AREA :

DNA

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs). After long primary transcript pri-miRNAs are processed to stem-looped pre- miRNAs by Drosha, pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22- nucleotide mature miRNAs . The mature miRNA then becomes a part of the RNA- Induced Silencing Complex (RISC) and can bind to the 3â€² UTR of the target mRNA.

FUNCTION :

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity). {UniProtKB:Q9UPY3}.; [Isoform 2]: More active than isoform 1 to process long double-stranded RNA into siRNAs. Responsible for the accumulation of endogenous siRNAs observed in mouse oocytes compared to somatic cells and it regulates meiotic spindle organization in female germline.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term use.

SPECIFICITY :

This antibody recognizes human, mouse and rat Dicer.

Image Name_1 :

qed-bioscience-anti-dicer-antibody-12532-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-dicer-antibody-12532-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-dicer-antibody-12532-3.png

Applications 3 :

ICC