Anti-HSF1 Antibody | QED Bioscience 12518 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-HSF1 Antibody

catalog :

12518

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

10H8

The same clone is also sold as:

OriGene: TA326414
Santa Cruz Biotechnology: sc-13516, sc-13516 X, sc-13516 AC, sc-13516 HRP
StressMarq Biosciences: SMc-118
Abcam: ab61382
Rockland Immunochemicals: 200-501-F50
Invitrogen: MA5-45128, MA5-45129, MA5-45130, MA5-45127, MA5-27688

reactivity :

human, mouse, rat, bovine

product information

CATALOG NUMBER :

12518

PRODUCT NAME :

Anti-HSF1 Antibody

NAME+SKU :

Anti-HSF1 Antibody (12518)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-HSF1 Rat Monoclonal Antibody

SPECIFICITY SHORT :

HSF1

SPECIES REACTIVITY :

Human, mouse, rat, raabbit, bovine, guinea pig, hamster, monkey

TARGET NAME :

HSF1

UNIPROT :

Q00613

LOCATION :

Nucleus , Cytoplasm , Nucleus, nucleoplasm , Cytoplasm, perinuclear region , Cytoplasm, cytoskeleton, spindle pole , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome , Chromosome, centromere, kinetochore

PROTEIN NAME :

Heat shock factor protein 1

ALTERNATIVE NAMES :

HSF 1, Heat shock transcription factor 1, HSTF 1

GENE NAME :

HSF1

ACCESSION NUMBER :

NP_005517

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

259.7

SKU SIZE 1 :

12518

APPLICATIONS :

WB, IP, ICC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-2ug/ml. A band of ~85 and/or ~95 kDa is detected. Immunocytochemistry: use at 10ug/ml. Immunoprecipitation: use at 12.5ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate

HOST :

Rat

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

10H8

IMMUNOGEN :

Recombinant mouse HSF-1

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up- regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. In higher eukaryotes, HSF1 is distributed diffusely in the cell cytoplasm and nucleus in unstressed cells. On exposure to heat shock or other stresses, HSF1 localizes to discrete nuclear granules; on recovery from stress, it returns to a diffuse nuclear- cytoplasmic distribution.

FUNCTION :

Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925, PubMed:18451878). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival (PubMed:18451878). Activation is reversible, and during the attenuation and rPubMed:10359787, PubMed:11447121, PubMed:11583998, PubMed:12659875, PubMed:12917326, PubMed:14707147, PubMed:15016915, PubMed:16278218, PubMed:17897941, PubMed:18451878, PubMed:1871105, PubMed:18794143, PubMed:1986252, PubMed:25963659, PubMed:26359349, PubMed:26727489, PubMed:26754925, PubMed:7623826, PubMed:7760831, PubMed:7935471, PubMed:8455624, PubMed:8940068, PubMed:8946918, PubMed:9121459, PubMed:9341107, PubMed:9499401, PubMed:9535852, PubMed:9727490}.; (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. {PubMed:27189267}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes an ~85 kDa protein (inactive form) in unstressed cell lysates and an ~95 kDa protein (active form) in heat-shocked cell lysates of human, mouse, rat, rabbit, bovine, guinea pig, hamster, and monkey.

Image Name_1 :

qed-bioscience-anti-hsf1-antibody-12518-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-hsf1-antibody-12518-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-hsf1-antibody-12518-3.png

Applications 3 :

ICC

Image Name_4 :

qed-bioscience-anti-hsf1-antibody-12518-4.png

Image Caption_4 :

Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

Applications 4 :

IHC

Image Name_5 :

qed-bioscience-anti-hsf1-antibody-12518-5.png

Image Caption_5 :

Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 µg). Lane 4: MCF7 lysate (10 µg). Lane 5: MCF7 lysate (20 µg). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

Applications 5 :

Western Blot