Anti-Chloride Channel Protein 3 (Clcn3) Antibody | QED Bioscience 11566 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Chloride Channel Protein 3 (Clcn3) Antibody

catalog :

11566

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S258-5

The same clone is also sold as:

Novus Biologicals: NBP2-59379
OriGene: TA326523

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11566

PRODUCT NAME :

Anti-Chloride Channel Protein 3 (Clcn3) Antibody

NAME+SKU :

Anti-Chloride Channel Protein 3 (Clcn3) Antibody (11566)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Chloride Channel Protein 3 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Chloride Channel Protein 3

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Chloride Channel Protein 3 (Clcn3)

UNIPROT :

P51792

LOCATION :

[Isoform 1]: Lysosome membrane , Late endosome membrane , Cell membrane , Early endosome membrane

PROTEIN NAME :

H(+)/Cl(-) exchange transporter 3

ALTERNATIVE NAMES :

Chloride channel protein 3, ClC-3, Chloride transporter ClC-3

GENE NAME :

Clcn3

ACCESSION NUMBER :

NP_445815.2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11566

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-2ug/ml. A band of ~90kDa is detected. Immunohistochemistry: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain membranes.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S258-5

IMMUNOGEN :

Synthetic peptide correspond- ing to aa 98-115 (cytoplasmic N-terminus) of rat Clcn3 (accession no. NP_445815.2). Host Species: Mouse

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Clcn3 mediates the exchange of chloride ions against protons and functions as an antiporter, contributing to the acidification of the endosome and synaptic vesicle lumen. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

FUNCTION :

[Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (By similarity). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity). {UniProtKB:P51790, PubMed:10915634}.; [Isoform 2]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C (PubMed:8155321). It could help neuronal cells to establish short-term memory (PubMed:8155321). {PubMed:10915634, PubMed:8155321}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rat Clcn3. It does not cross-react with Clcn4 or Clcn5.

Image Name_1 :

qed-bioscience-anti-chloride-channel-protein-3-clcn3-antibody-11566-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-chloride-channel-protein-3-clcn3-antibody-11566-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-chloride-channel-protein-3-clcn3-antibody-11566-3.png

Applications 3 :

ICC