Anti-Sodium Iodide Symporter Antibody | QED Bioscience 11563 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Sodium Iodide Symporter Antibody

catalog :

11563

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

FP5

The same clone is also sold as:

OriGene: TA309483
Invitrogen: MA5-45494, MA5-45492, MA5-45493
Rockland Immunochemicals: 200-301-G50
Novus Biologicals: NBP1-70342
Abcam: ab242007
StressMarq Biosciences: SMC-391

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11563

PRODUCT NAME :

Anti-Sodium Iodide Symporter Antibody

NAME+SKU :

Anti-Sodium Iodide Symporter Antibody (11563)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Sodium Iodine Symporter Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Sodium Iodine Symporter

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Sodium Iodide Symporter

UNIPROT :

Q92911

LOCATION :

Membrane; Multi-pass membrane protein.

PROTEIN NAME :

Sodium/iodide cotransporter

ALTERNATIVE NAMES :

Na(+/I(- cotransporter, Sodium-iodide symporter, Na(+/I(- symporter, Solute carrier family 5 member 5

GENE NAME :

SLC5A5

ACCESSION NUMBER :

NP_000444.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11563

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. A major band of ~97kDa is detected. Depending on the sample, some minor bands may be detected as well.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

FP5

IMMUNOGEN :

Fusion protein consisting of aa468-643 of human NIS attached to mannose binding protein (MBP).

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells. This is an important step in the process of iodide organification and the formation of triiodothyronine and thyroxine. The importance of NIS is related to its role in thyroid function, the pathogenesis of thyroid disease, and its expression (or lack of expression) in thyroid malignancies. It has also been suggested that NIS may represent an autoantigen in autoimmune disease of the thyroid.

FUNCTION :

Mediates iodide uptake in the thyroid gland.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse and rat NIS.Immunohistochemistry: use at 1-10ug/ml. Positive control: Graves disease-thyroid tissue.These are recommended concentrations; enduser should determine optimal concentrations for their

Image Name_1 :

qed-bioscience-anti-sodium-iodide-symporter-antibody-11563-1.png

Applications 1 :

IHC

Image Name_2 :

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Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-sodium-iodide-symporter-antibody-11563-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-sodium-iodide-symporter-antibody-11563-4.png

Image Caption_4 :

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

Applications 4 :

IHC

Image Name_5 :

qed-bioscience-anti-sodium-iodide-symporter-antibody-11563-5.png

Image Caption_5 :

Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000.

Applications 5 :

Western Blot