Anti-Cavbeta2 Ca+2 Channel Antibody | QED Bioscience 11524 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Cavbeta2 Ca+2 Channel Antibody

catalog :

11524

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S8B-1

The same clone is also sold as:

Rockland Immunochemicals: 200-301-F06
OriGene: TA309311
MyBioSource: MBS190548

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11524

PRODUCT NAME :

Anti-Cavbeta2 Ca+2 Channel Antibody

NAME+SKU :

Anti-Cavbeta2 Ca+2 Channel Antibody (11524)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Cavß Ca+2 Channel Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Cavß Ca+2 Channel

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Cavbeta2 Ca+2 Channel

UNIPROT :

Q8VGC3

LOCATION :

Cell membrane, sarcolemma , Peripheral membrane protein , Cytoplasmic side

PROTEIN NAME :

Voltage-dependent L-type calcium channel subunit beta-2

ALTERNATIVE NAMES :

CAB2, Calcium channel voltage-dependent subunit beta 2

GENE NAME :

Cacnb2

ACCESSION NUMBER :

NP_446303

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11524

APPLICATIONS :

WB, IHC, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~78kDa is detected. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S8b-1

IMMUNOGEN :

Synthetic peptide corresponding to aa 189-205 of rat Cavbeta2 (RSPKPSANSVTSPHSKE) (accession no. NP_446303).

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cavbeta subunits are involved in the transport of the pore-forming alpha1 subunit to the plasma membrane. They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore- forming subunit to the target membrane.

FUNCTION :

The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. {PubMed:11604404, PubMed:12042350, PubMed:1370480}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rat Cavbeta2. It does not cross-react with Cav beta1, Cav beta3, or Cav beta4.

Image Name_1 :

qed-bioscience-anti-cavbeta2-ca+2-channel-antibody-11524-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-cavbeta2-ca+2-channel-antibody-11524-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-cavbeta2-ca+2-channel-antibody-11524-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-cavbeta2-ca+2-channel-antibody-11524-4.png

Image Caption_4 :

Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot