Anti-TrpV3 Cation Channel Antibody | QED Bioscience 11520 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-TrpV3 Cation Channel Antibody

catalog :

11520

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S15-4

The same clone is also sold as:

OriGene: TA326598
Rockland Immunochemicals: 200-301-G58
Novus Biologicals: NBP2-12909

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11520

PRODUCT NAME :

Anti-TrpV3 Cation Channel Antibody

NAME+SKU :

Anti-TrpV3 Cation Channel Antibody (11520)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-TrpV3 Cation Channel Mouse Monoclonal Antibody

SPECIFICITY SHORT :

TrpV3 Cation Channel

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

TrpV3 Cation Channel

UNIPROT :

Q4QYD9

PROTEIN NAME :

Heat sensitive channel TRPV3

ACCESSION NUMBER :

NP_001020928

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11520

APPLICATIONS :

WB, IHC, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~70kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml Immunofluorescence: use at 1-10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: COS cell lysate transiently transfected with TrpM7.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

S15-4

IMMUNOGEN :

Synthetic peptide corresponding to aa 458-474 (cytoplasmic C-terminus) of rat TrpV3 (accession no. NP_001020928).

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The TrpV3 protein belongs to a family of nonselective cation channels that function in a variety of processes including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin and are activated at distinct physiological temperatures. The TrpV3 channel is activated at temperatures between 22 and 40 C. The TrpV3 gene is in close proximity to the TrpV1 gene on chromosome 17, and studies suggest that the corresponding two proteins associate with each other to form heteromeric channels.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rat TrpV3.

Image Name_1 :

qed-bioscience-anti-trpv3-cation-channel-antibody-11520-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-trpv3-cation-channel-antibody-11520-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-trpv3-cation-channel-antibody-11520-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-trpv3-cation-channel-antibody-11520-4.png

Image Caption_4 :

Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (11520). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (11520) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot