Anti-KCNQ4 K+ Channel Antibody | QED Bioscience 11512 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-KCNQ4 K+ Channel Antibody

catalog :

11512

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S43-6

The same clone is also sold as:

OriGene: TA326401
Rockland Immunochemicals: 200-301-F84
Novus Biologicals: NBP2-12899

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11512

PRODUCT NAME :

Anti-KCNQ4 K+ Channel Antibody

NAME+SKU :

Anti-KCNQ4 K+ Channel Antibody (11512)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-KCNQ4 K+ Channel Mouse Monoclonal Antibody

SPECIFICITY SHORT :

KCNQ4 K+ Channel

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

KCNQ4 K+ Channel

UNIPROT :

P56696

LOCATION :

Basal cell membrane; Multi-pass membrane protein. Note=Situated at the basal membrane of cochlear outer hair cells.

PROTEIN NAME :

Potassium voltage-gated channel subfamily KQT member 4

ALTERNATIVE NAMES :

KQT-like 4, Potassium channel subunit alpha KvLQT4, Voltage-gated potassium channel subunit Kv7.4

GENE NAME :

KCNQ4

ACCESSION NUMBER :

NP_004691.2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11512

APPLICATIONS :

WB, IHC, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~77kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml These are recommended concentrations; enduser should determine optimal concentrations for their applications. Positive control: COS cell lysate transiently expressing KCNQ4.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S43-6

IMMUNOGEN :

Fusion protein aa 2-77 of human KCNQ4 (K+7.4, KvLQT4, accession number P56696).

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The protein coded for by the KCNQ4 gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug.

FUNCTION :

Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse and rat KCNQ4.

Image Name_1 :

qed-bioscience-anti-kcnq4-k+-channel-antibody-11512-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-kcnq4-k+-channel-antibody-11512-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-kcnq4-k+-channel-antibody-11512-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-kcnq4-k+-channel-antibody-11512-4.png

Image Caption_4 :

Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot