Anti-KCNQ1 K+ Channel Antibody | QED Bioscience 11510 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-KCNQ1 K+ Channel Antibody

catalog :

11510

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S37A-10

The same clone is also sold as:

OriGene: TA326344
Novus Biologicals: NBP2-12897
Rockland Immunochemicals: 200-301-F82

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11510

PRODUCT NAME :

Anti-KCNQ1 K+ Channel Antibody

NAME+SKU :

Anti-KCNQ1 K+ Channel Antibody (11510)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-KCNQ1 K+ Channel Mouse Monoclonal Antibody

SPECIFICITY SHORT :

KCNQ1 K+ Channel

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

KCNQ1 K+ Channel

UNIPROT :

P51787

LOCATION :

Cell membrane , Cytoplasmic vesicle membrane , Early endosome , Membrane raft , Endoplasmic reticulum , Basolateral cell membrane

PROTEIN NAME :

Potassium voltage-gated channel subfamily KQT member 1

ALTERNATIVE NAMES :

IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1, KQT-like 1, Voltage-gated potassium channel subunit Kv7.1

GENE NAME :

KCNQ1

ACCESSION NUMBER :

NP_000209.2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11510

APPLICATIONS :

WB, IHC, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~75kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. These are recommended concentrations; enduser should determine optimal concentrations for their applications. Positive control: COS cell lysate transiently expressing KCNQ1.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S37A-10

IMMUNOGEN :

Fusion protein aa 2-101 of human KCNQ1 (K+7.1, KvLQT1, accession number P51787).

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. â€“+7.1 (KvLQT1) is a potassium channel protein coded for by the gene KCNQ1. K+7.1 is present in cell membranes of cardiac muscle tissue and in inner ear neurons among other tissues. In cardiac cells, K+7.1 mediates the I. (or slow delayed rectifying K3) current that contributes to repolarization of the cell, terminating the cardiac potential and, thereby, the heartâ€™s contractions.

FUNCTION :

Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (PubMed:10646604, PubMed:25441029). Associates with KCNE beta subunits that modulates current kinetics (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505, PubMed:19687231). Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505, PubMed:25441029). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed:10713961). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed:10646604). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed:19687231). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed:12324418). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057). Binds with phosphatidylinositol 4,5-bisphosphate (PubMed:25037568). {UniProtKB:P97414, UniProtKB:Q9Z0N7, PubMed:10646604, PubMed:10713961, PubMed:11101505, PubMed:12324418, PubMed:19687231, PubMed:24855057, PubMed:25037568, PubMed:8900283, PubMed:9108097, PubMed:9312006}.; [Isoform 2]: Non-functional alone but modulatory when coexpressed with the full-length isoform 1. {PubMed:9305853}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse and rat KCNQ1.

Image Name_1 :

qed-bioscience-anti-kcnq1-k+-channel-antibody-11510-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-kcnq1-k+-channel-antibody-11510-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-kcnq1-k+-channel-antibody-11510-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-kcnq1-k+-channel-antibody-11510-4.png

Image Caption_4 :

Western Blot analysis of Hamster T-CHO cell lysate showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone N37A/10 (11510). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody (11510) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot

Image Name_5 :

qed-bioscience-anti-kcnq1-k+-channel-antibody-11510-5.png

Image Caption_5 :

Western Blot analysis of Human Cell lysates showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone N37A/10 (11510). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody (11510) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 5 :

Western Blot