Anti-HCN4 Cyclic nucleotide-gated channel Antibody | QED Bioscience 11509 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-HCN4 Cyclic nucleotide-gated channel Antibody

catalog :

11509

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S114-10

The same clone is also sold as:

Novus Biologicals: NBP2-12910
MyBioSource: MBS800358
Rockland Immunochemicals: 200-301-F43
OriGene: TA326545

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11509

PRODUCT NAME :

Anti-HCN4 Cyclic nucleotide-gated channel Antibody

NAME+SKU :

Anti-HCN4 Cyclic nucleotide-gated channel Antibody (11509)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-HCN4 Cyclic nucleotide-gated Channel Mouse Monoclonal Antibody

SPECIFICITY SHORT :

HCN4 Cyclic nucleotide-gated Channel

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

HCN4 Cyclic nucleotide-gated channel

UNIPROT :

Q9JKA7

LOCATION :

Cell membrane , Multi-pass membrane protein

PROTEIN NAME :

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

GENE NAME :

Hcn4

ACCESSION NUMBER :

NP_067690.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

11509

APPLICATIONS :

WB, IHC, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-10ug/ml. A band of ~1300kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml Immunofluorescence: use at 1-10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: COS cell lysate transiently transfected with HCN4.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S114-10

IMMUNOGEN :

Fusion protein aa 1019-1108 (cytoplasmic C-terminus) of rat HCN4 (accession no. Q9JKA7).

RESEARCH AREA :

Ion Channels

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN4, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain.

FUNCTION :

Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih) (By similarity). May mediate responses to sour stimuli. {PubMed:11675786}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rat HCN4. It does not cross-react with other HCNs.

Image Name_1 :

qed-bioscience-anti-hcn4-cyclic-nucleotide-gated-channel-antibody-11509-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-hcn4-cyclic-nucleotide-gated-channel-antibody-11509-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-hcn4-cyclic-nucleotide-gated-channel-antibody-11509-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-hcn4-cyclic-nucleotide-gated-channel-antibody-11509-4.png

Image Caption_4 :

Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot