Anti-TRAP1/Hsp75 Antibody | QED Bioscience 11136 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-TRAP1/Hsp75 Antibody

catalog :

11136

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Trap1-6

The same clone is also sold as:

OriGene: TA326389
Novus Biologicals: NB300-555
Rockland Immunochemicals: 200-301-G53
Invitrogen: MA1-010
Abcam: ab2721

reactivity :

human

product information

CATALOG NUMBER :

11136

PRODUCT NAME :

Anti-TRAP1/Hsp75 Antibody

NAME+SKU :

Anti-TRAP1/Hsp75 Antibody (11136)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-TRAP1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

TRAP1

SPECIES REACTIVITY :

Human

TARGET NAME :

TRAP1/Hsp75

UNIPROT :

Q12931

LOCATION :

Mitochondrion , Mitochondrion inner membrane , Mitochondrion matrix

PROTEIN NAME :

Heat shock protein 75 kDa, mitochondrial

ALTERNATIVE NAMES :

HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1

GENE NAME :

TRAP1

ACCESSION NUMBER :

NP_057376.2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

298.9

SKU SIZE 1 :

11136

APPLICATIONS :

WB, ICC, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. A band of ~75kDa is detected. Immunocytochemistry: use at 0.1-1.0ug/ml. Immunofluorescence: use at 1-10ug/ml. Positive control: HeLa cells or lysate These are recommended concentrations; enduser should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

Trap1-6

IMMUNOGEN :

Recombinant human TRAP1

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

The molecular chaperone family of 90kDa heat shock proteins (Hsp90) is a highly conserved protein family that plays an important physiological role. Hsp90 is best known for its association with signal transduction processes. Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a recently cloned homolog of Hsp90. Like Hsp90, TRAP1 is associated with numerous proteins that are involved in multiple pathways. Data indicates that TRAP1 is a mitochondrial matrix protein: it is localized in mitochondria of mammalian cells and contains a mitochondrial targeting presequence.

FUNCTION :

Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. {PubMed:23525905, PubMed:23564345, PubMed:23747254}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes human TRAP1. Other species have not been investigated.

Image Name_1 :

qed-bioscience-anti-trap1-hsp75-antibody-11136-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-trap1-hsp75-antibody-11136-2.png

Applications 2 :

Western Blot